Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3263 and BT3264 overlap by 14 nucleotides BT3264 and BT3265 are separated by 88 nucleotides
BT3263: BT3263 - putative deoxyribose-phosphate aldolase (NCBI ptt file), at 4,167,078 to 4,167,980
BT3263
BT3264: BT3264 - conserved hypothetical protein (NCBI ptt file), at 4,167,967 to 4,168,305
BT3264
BT3265: BT3265 - putative D-tyrosyl-tRNA deacylase (NCBI ptt file), at 4,168,394 to 4,168,846
BT3265
Position (kb)
4167
4168
4169 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4166.978 kb on - strand at 4166.978 kb on - strand at 4166.981 kb on + strand at 4166.995 kb on - strand at 4167.000 kb on - strand at 4167.055 kb on + strand at 4167.055 kb on + strand at 4167.071 kb on + strand at 4167.072 kb on - strand at 4167.072 kb on - strand at 4167.108 kb on - strand at 4167.198 kb on - strand, within BT3263 at 4167.202 kb on + strand, within BT3263 at 4167.203 kb on - strand, within BT3263 at 4167.280 kb on + strand, within BT3263 at 4167.384 kb on + strand, within BT3263 at 4167.385 kb on - strand, within BT3263 at 4167.441 kb on + strand, within BT3263 at 4167.474 kb on + strand, within BT3263 at 4167.537 kb on - strand, within BT3263 at 4167.542 kb on + strand, within BT3263 at 4167.542 kb on + strand, within BT3263 at 4167.542 kb on + strand, within BT3263 at 4167.542 kb on + strand, within BT3263 at 4167.542 kb on + strand, within BT3263 at 4167.542 kb on + strand, within BT3263 at 4167.543 kb on - strand, within BT3263 at 4167.547 kb on - strand, within BT3263 at 4167.644 kb on + strand, within BT3263 at 4167.645 kb on - strand, within BT3263 at 4167.668 kb on + strand, within BT3263 at 4167.668 kb on + strand, within BT3263 at 4167.669 kb on - strand, within BT3263 at 4167.700 kb on - strand, within BT3263 at 4167.900 kb on + strand at 4167.926 kb on + strand at 4167.926 kb on + strand at 4167.928 kb on + strand at 4167.949 kb on + strand at 4167.950 kb on - strand at 4168.005 kb on + strand, within BT3264 at 4168.006 kb on - strand, within BT3264 at 4168.014 kb on + strand, within BT3264 at 4168.090 kb on - strand, within BT3264 at 4168.105 kb on + strand, within BT3264 at 4168.117 kb on + strand, within BT3264 at 4168.216 kb on + strand, within BT3264 at 4168.277 kb on - strand at 4168.305 kb on + strand at 4168.306 kb on - strand at 4168.330 kb on - strand at 4168.396 kb on - strand at 4168.417 kb on + strand at 4168.417 kb on + strand at 4168.417 kb on + strand at 4168.418 kb on - strand at 4168.423 kb on + strand at 4168.423 kb on + strand at 4168.423 kb on + strand at 4168.423 kb on + strand at 4168.427 kb on - strand at 4168.427 kb on - strand at 4168.530 kb on + strand, within BT3265 at 4168.544 kb on + strand, within BT3265 at 4168.665 kb on - strand, within BT3265 at 4168.698 kb on - strand, within BT3265 at 4168.721 kb on + strand, within BT3265 at 4168.721 kb on + strand, within BT3265 at 4168.723 kb on + strand, within BT3265 at 4168.724 kb on - strand, within BT3265 at 4168.757 kb on + strand, within BT3265 at 4168.808 kb on + strand at 4168.823 kb on + strand at 4168.832 kb on + strand at 4168.832 kb on + strand at 4168.838 kb on + strand at 4168.838 kb on + strand at 4168.859 kb on + strand at 4168.874 kb on + strand at 4168.874 kb on + strand at 4168.932 kb on - strand at 4168.941 kb on - strand at 4168.944 kb on + strand at 4168.948 kb on - strand at 4168.951 kb on + strand at 4168.959 kb on - strand at 4169.009 kb on + strand at 4169.009 kb on + strand at 4169.009 kb on + strand at 4169.010 kb on - strand at 4169.013 kb on + strand at 4169.014 kb on - strand at 4169.014 kb on - strand at 4169.016 kb on + strand at 4169.030 kb on + strand at 4169.030 kb on + strand at 4169.030 kb on + strand at 4169.031 kb on - strand at 4169.031 kb on - strand at 4169.072 kb on - strand at 4169.083 kb on + strand at 4169.084 kb on - strand at 4169.084 kb on - strand at 4169.137 kb on + strand at 4169.137 kb on + strand at 4169.145 kb on + strand at 4169.289 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 4,166,978 - -1.6 4,166,978 - +0.4 4,166,981 + -0.7 4,166,995 - +0.5 4,167,000 - -1.9 4,167,055 + -2.0 4,167,055 + +1.8 4,167,071 + -0.2 4,167,072 - -0.1 4,167,072 - -0.0 4,167,108 - -0.4 4,167,198 - BT3263 0.13 -1.6 4,167,202 + BT3263 0.14 +0.4 4,167,203 - BT3263 0.14 -1.0 4,167,280 + BT3263 0.22 +0.5 4,167,384 + BT3263 0.34 -0.2 4,167,385 - BT3263 0.34 -0.5 4,167,441 + BT3263 0.40 -2.8 4,167,474 + BT3263 0.44 -1.3 4,167,537 - BT3263 0.51 +0.1 4,167,542 + BT3263 0.51 -1.0 4,167,542 + BT3263 0.51 +0.8 4,167,542 + BT3263 0.51 -0.2 4,167,542 + BT3263 0.51 -0.1 4,167,542 + BT3263 0.51 -0.0 4,167,542 + BT3263 0.51 -1.6 4,167,543 - BT3263 0.51 +1.0 4,167,547 - BT3263 0.52 +0.0 4,167,644 + BT3263 0.63 +0.8 4,167,645 - BT3263 0.63 -1.5 4,167,668 + BT3263 0.65 +0.2 4,167,668 + BT3263 0.65 -2.0 4,167,669 - BT3263 0.65 -0.8 4,167,700 - BT3263 0.69 -0.4 4,167,900 + +0.1 4,167,926 + +0.2 4,167,926 + +0.1 4,167,928 + +0.4 4,167,949 + -0.5 4,167,950 - +0.6 4,168,005 + BT3264 0.11 -1.3 4,168,006 - BT3264 0.12 -1.5 4,168,014 + BT3264 0.14 -1.9 4,168,090 - BT3264 0.36 -1.1 4,168,105 + BT3264 0.41 -2.7 4,168,117 + BT3264 0.44 +1.0 4,168,216 + BT3264 0.73 -0.5 4,168,277 - +1.8 4,168,305 + -2.2 4,168,306 - -0.4 4,168,330 - -0.4 4,168,396 - +0.8 4,168,417 + +0.2 4,168,417 + -1.9 4,168,417 + +0.9 4,168,418 - +0.8 4,168,423 + +0.8 4,168,423 + -0.5 4,168,423 + -1.5 4,168,423 + +0.1 4,168,427 - -1.6 4,168,427 - -0.5 4,168,530 + BT3265 0.30 +0.3 4,168,544 + BT3265 0.33 -0.6 4,168,665 - BT3265 0.60 -1.0 4,168,698 - BT3265 0.67 +0.8 4,168,721 + BT3265 0.72 +2.6 4,168,721 + BT3265 0.72 -1.5 4,168,723 + BT3265 0.73 +1.0 4,168,724 - BT3265 0.73 +0.8 4,168,757 + BT3265 0.80 -0.2 4,168,808 + -1.2 4,168,823 + -0.2 4,168,832 + -0.4 4,168,832 + -1.9 4,168,838 + -1.5 4,168,838 + +0.7 4,168,859 + -1.0 4,168,874 + -0.9 4,168,874 + +0.4 4,168,932 - -0.8 4,168,941 - +0.2 4,168,944 + -2.4 4,168,948 - +0.4 4,168,951 + -1.4 4,168,959 - -0.5 4,169,009 + -1.8 4,169,009 + +0.2 4,169,009 + -0.5 4,169,010 - -0.2 4,169,013 + -1.9 4,169,014 - -0.0 4,169,014 - -2.4 4,169,016 + -0.4 4,169,030 + -0.7 4,169,030 + +1.4 4,169,030 + -2.0 4,169,031 - +0.2 4,169,031 - +0.8 4,169,072 - -1.4 4,169,083 + -0.5 4,169,084 - -1.4 4,169,084 - -2.0 4,169,137 + -0.2 4,169,137 + +0.0 4,169,145 + +1.8 4,169,289 - -0.6
Or see this region's nucleotide sequence