Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0301 and BT0302 are separated by 15 nucleotides BT0302 and BT0303 are separated by 149 nucleotides BT0303 and BT0304 are separated by 271 nucleotides
BT0301: BT0301 - ATP-binding transport protein natA (Na+ ABC transporter) (NCBI ptt file), at 358,006 to 359,517
BT0301
BT0302: BT0302 - hypothetical protein (NCBI ptt file), at 359,533 to 360,234
BT0302
BT0303: BT0303 - putative patatin-like phospholipase (NCBI ptt file), at 360,384 to 362,600
BT0303
BT0304: BT0304 - acriflavine resistance protein A precursor (NCBI ptt file), at 362,872 to 364,107
BT0304
Position (kb) 360
361
362
363 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 359.388 kb on - strand at 359.395 kb on - strand at 359.447 kb on - strand at 359.488 kb on + strand at 359.750 kb on + strand, within BT0302 at 359.815 kb on - strand, within BT0302 at 359.819 kb on - strand, within BT0302 at 359.821 kb on - strand, within BT0302 at 359.901 kb on - strand, within BT0302 at 359.979 kb on + strand, within BT0302 at 359.979 kb on + strand, within BT0302 at 360.020 kb on + strand, within BT0302 at 360.021 kb on - strand, within BT0302 at 360.030 kb on + strand, within BT0302 at 360.346 kb on - strand at 360.350 kb on - strand at 360.351 kb on + strand at 360.362 kb on - strand at 360.386 kb on - strand at 360.394 kb on + strand at 360.471 kb on - strand at 360.479 kb on + strand at 360.479 kb on + strand at 360.498 kb on - strand at 360.522 kb on + strand at 360.553 kb on + strand at 360.558 kb on - strand at 360.724 kb on + strand, within BT0303 at 360.757 kb on + strand, within BT0303 at 360.846 kb on - strand, within BT0303 at 360.846 kb on - strand, within BT0303 at 360.857 kb on + strand, within BT0303 at 360.932 kb on + strand, within BT0303 at 360.935 kb on - strand, within BT0303 at 360.939 kb on + strand, within BT0303 at 361.030 kb on + strand, within BT0303 at 361.105 kb on + strand, within BT0303 at 361.112 kb on - strand, within BT0303 at 361.172 kb on + strand, within BT0303 at 361.216 kb on + strand, within BT0303 at 361.277 kb on + strand, within BT0303 at 361.397 kb on - strand, within BT0303 at 361.424 kb on - strand, within BT0303 at 361.569 kb on - strand, within BT0303 at 361.633 kb on + strand, within BT0303 at 361.633 kb on + strand, within BT0303 at 361.765 kb on - strand, within BT0303 at 361.781 kb on - strand, within BT0303 at 361.781 kb on - strand, within BT0303 at 361.985 kb on + strand, within BT0303 at 361.986 kb on - strand, within BT0303 at 362.068 kb on - strand, within BT0303 at 362.397 kb on - strand at 362.416 kb on - strand at 362.469 kb on - strand at 362.537 kb on - strand at 362.650 kb on - strand at 362.725 kb on - strand at 362.855 kb on - strand at 362.934 kb on - strand at 362.937 kb on - strand at 363.058 kb on + strand, within BT0304 at 363.058 kb on + strand, within BT0304 at 363.095 kb on + strand, within BT0304 at 363.096 kb on - strand, within BT0304 at 363.277 kb on - strand, within BT0304 at 363.290 kb on - strand, within BT0304 at 363.341 kb on - strand, within BT0304 at 363.442 kb on - strand, within BT0304 at 363.505 kb on - strand, within BT0304 at 363.509 kb on + strand, within BT0304
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 359,388 - -0.5 359,395 - -3.8 359,447 - -1.3 359,488 + +1.7 359,750 + BT0302 0.31 -1.3 359,815 - BT0302 0.40 +1.9 359,819 - BT0302 0.41 +0.5 359,821 - BT0302 0.41 -1.1 359,901 - BT0302 0.52 +0.1 359,979 + BT0302 0.64 -0.7 359,979 + BT0302 0.64 -1.0 360,020 + BT0302 0.69 -0.8 360,021 - BT0302 0.70 -0.2 360,030 + BT0302 0.71 -1.7 360,346 - -0.2 360,350 - -2.1 360,351 + -0.7 360,362 - +1.7 360,386 - -0.5 360,394 + -2.0 360,471 - -1.7 360,479 + -2.0 360,479 + -1.3 360,498 - -0.3 360,522 + -2.6 360,553 + +0.3 360,558 - -0.7 360,724 + BT0303 0.15 -2.9 360,757 + BT0303 0.17 +1.3 360,846 - BT0303 0.21 -2.9 360,846 - BT0303 0.21 -0.3 360,857 + BT0303 0.21 -0.3 360,932 + BT0303 0.25 -2.4 360,935 - BT0303 0.25 -1.5 360,939 + BT0303 0.25 -2.6 361,030 + BT0303 0.29 -4.7 361,105 + BT0303 0.33 -0.6 361,112 - BT0303 0.33 -1.0 361,172 + BT0303 0.36 -1.5 361,216 + BT0303 0.38 -3.0 361,277 + BT0303 0.40 +1.3 361,397 - BT0303 0.46 -0.7 361,424 - BT0303 0.47 +0.2 361,569 - BT0303 0.53 +0.6 361,633 + BT0303 0.56 -0.7 361,633 + BT0303 0.56 -1.0 361,765 - BT0303 0.62 -1.2 361,781 - BT0303 0.63 -1.7 361,781 - BT0303 0.63 +1.3 361,985 + BT0303 0.72 -0.7 361,986 - BT0303 0.72 -0.7 362,068 - BT0303 0.76 +0.5 362,397 - -2.0 362,416 - -2.0 362,469 - -0.7 362,537 - -1.7 362,650 - +1.1 362,725 - +1.0 362,855 - -1.9 362,934 - +0.7 362,937 - +3.0 363,058 + BT0304 0.15 -0.5 363,058 + BT0304 0.15 +0.7 363,095 + BT0304 0.18 +0.0 363,096 - BT0304 0.18 +2.0 363,277 - BT0304 0.33 +2.1 363,290 - BT0304 0.34 -0.5 363,341 - BT0304 0.38 -0.5 363,442 - BT0304 0.46 +0.1 363,505 - BT0304 0.51 +0.9 363,509 + BT0304 0.52 -0.3
Or see this region's nucleotide sequence