Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0298 and BT0299 are separated by 48 nucleotides BT0299 and BT0300 are separated by 2 nucleotides
BT0298: BT0298 - cation efflux system protein (outer membrane protein) (NCBI ptt file), at 350,578 to 351,639
BT0298
BT0299: BT0299 - cation efflux system (AcrB/AcrD/AcrF family) (NCBI ptt file), at 351,688 to 354,804
BT0299
BT0300: BT0300 - cation efflux system (AcrB/AcrD/AcrF family) (NCBI ptt file), at 354,807 to 358,001
BT0300
Position (kb) 351
352
353
354
355 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 350.730 kb on + strand, within BT0298 at 350.756 kb on - strand, within BT0298 at 350.756 kb on - strand, within BT0298 at 350.864 kb on + strand, within BT0298 at 351.007 kb on - strand, within BT0298 at 351.172 kb on + strand, within BT0298 at 351.185 kb on - strand, within BT0298 at 351.247 kb on - strand, within BT0298 at 351.507 kb on - strand, within BT0298 at 351.539 kb on - strand at 351.539 kb on - strand at 351.638 kb on - strand at 351.654 kb on - strand at 351.655 kb on + strand at 351.656 kb on - strand at 351.656 kb on - strand at 351.740 kb on + strand at 351.770 kb on + strand at 351.775 kb on + strand at 351.814 kb on - strand at 351.888 kb on + strand at 351.969 kb on - strand at 351.973 kb on + strand at 351.973 kb on + strand at 351.974 kb on - strand at 351.974 kb on - strand at 351.975 kb on + strand at 352.050 kb on + strand, within BT0299 at 352.050 kb on + strand, within BT0299 at 352.128 kb on + strand, within BT0299 at 352.166 kb on - strand, within BT0299 at 352.239 kb on + strand, within BT0299 at 352.259 kb on - strand, within BT0299 at 352.261 kb on - strand, within BT0299 at 352.294 kb on - strand, within BT0299 at 352.363 kb on + strand, within BT0299 at 352.363 kb on + strand, within BT0299 at 352.494 kb on + strand, within BT0299 at 352.582 kb on - strand, within BT0299 at 352.625 kb on + strand, within BT0299 at 352.794 kb on - strand, within BT0299 at 352.836 kb on + strand, within BT0299 at 352.900 kb on - strand, within BT0299 at 352.969 kb on - strand, within BT0299 at 353.013 kb on + strand, within BT0299 at 353.056 kb on - strand, within BT0299 at 353.056 kb on - strand, within BT0299 at 353.067 kb on + strand, within BT0299 at 353.224 kb on - strand, within BT0299 at 353.261 kb on - strand, within BT0299 at 353.262 kb on + strand, within BT0299 at 353.474 kb on + strand, within BT0299 at 353.474 kb on + strand, within BT0299 at 353.475 kb on - strand, within BT0299 at 353.477 kb on - strand, within BT0299 at 353.477 kb on - strand, within BT0299 at 353.510 kb on + strand, within BT0299 at 353.513 kb on - strand, within BT0299 at 353.731 kb on - strand, within BT0299 at 353.810 kb on + strand, within BT0299 at 353.829 kb on + strand, within BT0299 at 353.919 kb on - strand, within BT0299 at 354.000 kb on - strand, within BT0299 at 354.091 kb on - strand, within BT0299 at 354.238 kb on + strand, within BT0299 at 354.329 kb on - strand, within BT0299 at 354.532 kb on - strand at 354.668 kb on + strand at 354.685 kb on - strand at 354.686 kb on + strand at 354.686 kb on + strand at 354.686 kb on + strand at 354.687 kb on - strand at 354.749 kb on - strand at 354.749 kb on - strand at 354.749 kb on - strand at 354.752 kb on - strand at 354.792 kb on + strand at 354.825 kb on - strand at 354.883 kb on - strand at 354.972 kb on + strand at 354.993 kb on - strand at 355.085 kb on + strand at 355.094 kb on + strand at 355.208 kb on + strand, within BT0300 at 355.211 kb on + strand, within BT0300 at 355.503 kb on + strand, within BT0300 at 355.606 kb on - strand, within BT0300
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 350,730 + BT0298 0.14 -1.5 350,756 - BT0298 0.17 +0.8 350,756 - BT0298 0.17 +0.3 350,864 + BT0298 0.27 +0.5 351,007 - BT0298 0.40 +1.3 351,172 + BT0298 0.56 +1.3 351,185 - BT0298 0.57 +1.0 351,247 - BT0298 0.63 -0.7 351,507 - BT0298 0.87 -0.6 351,539 - -2.5 351,539 - -3.2 351,638 - -1.6 351,654 - +1.3 351,655 + -2.1 351,656 - -1.0 351,656 - -2.2 351,740 + -0.5 351,770 + -0.6 351,775 + -1.3 351,814 - +0.5 351,888 + -2.2 351,969 - -0.8 351,973 + -0.3 351,973 + -0.1 351,974 - -1.7 351,974 - -1.7 351,975 + -0.3 352,050 + BT0299 0.12 -0.0 352,050 + BT0299 0.12 -0.3 352,128 + BT0299 0.14 +1.3 352,166 - BT0299 0.15 +1.3 352,239 + BT0299 0.18 -1.9 352,259 - BT0299 0.18 +0.6 352,261 - BT0299 0.18 +0.2 352,294 - BT0299 0.19 -0.1 352,363 + BT0299 0.22 -1.7 352,363 + BT0299 0.22 +0.1 352,494 + BT0299 0.26 -1.0 352,582 - BT0299 0.29 -1.3 352,625 + BT0299 0.30 -0.2 352,794 - BT0299 0.35 -1.2 352,836 + BT0299 0.37 +1.7 352,900 - BT0299 0.39 -0.4 352,969 - BT0299 0.41 -0.5 353,013 + BT0299 0.43 -0.3 353,056 - BT0299 0.44 +0.1 353,056 - BT0299 0.44 +1.0 353,067 + BT0299 0.44 -1.4 353,224 - BT0299 0.49 +1.6 353,261 - BT0299 0.50 +0.8 353,262 + BT0299 0.50 +0.4 353,474 + BT0299 0.57 +0.3 353,474 + BT0299 0.57 -1.5 353,475 - BT0299 0.57 -0.3 353,477 - BT0299 0.57 +0.7 353,477 - BT0299 0.57 -0.9 353,510 + BT0299 0.58 +1.5 353,513 - BT0299 0.59 +1.6 353,731 - BT0299 0.66 +0.9 353,810 + BT0299 0.68 -1.0 353,829 + BT0299 0.69 +0.9 353,919 - BT0299 0.72 -0.9 354,000 - BT0299 0.74 +0.8 354,091 - BT0299 0.77 -0.8 354,238 + BT0299 0.82 +0.4 354,329 - BT0299 0.85 +0.8 354,532 - -0.2 354,668 + +2.3 354,685 - -1.4 354,686 + -0.6 354,686 + -0.5 354,686 + -0.9 354,687 - -0.1 354,749 - +1.3 354,749 - +0.1 354,749 - -0.7 354,752 - +0.3 354,792 + -1.7 354,825 - -0.2 354,883 - -1.9 354,972 + +1.3 354,993 - -2.0 355,085 + -0.3 355,094 + -1.3 355,208 + BT0300 0.13 -2.8 355,211 + BT0300 0.13 -0.1 355,503 + BT0300 0.22 -0.3 355,606 - BT0300 0.25 +1.3
Or see this region's nucleotide sequence