Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07715 and MMP_RS07720 are separated by 12 nucleotides MMP_RS07720 and MMP_RS07725 are separated by 18 nucleotides MMP_RS07725 and MMP_RS07730 are separated by 23 nucleotides MMP_RS07730 and MMP_RS07735 are separated by 16 nucleotides
MMP_RS07715: MMP_RS07715 - YfcE family phosphodiesterase, at 1,462,031 to 1,462,522
_RS07715
MMP_RS07720: MMP_RS07720 - 4Fe-4S binding protein, at 1,462,535 to 1,462,951
_RS07720
MMP_RS07725: MMP_RS07725 - 4Fe-4S dicluster domain-containing protein, at 1,462,970 to 1,463,473
_RS07725
MMP_RS07730: MMP_RS07730 - pyruvate synthase subunit beta, at 1,463,497 to 1,464,393
_RS07730
MMP_RS07735: MMP_RS07735 - 2-ketoisovalerate ferredoxin oxidoreductase subunit alpha, at 1,464,410 to 1,465,573
_RS07735
Position (kb) 1463
1464
1465 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1462.559 kb on + strand at 1462.597 kb on - strand, within MMP_RS07720 at 1462.605 kb on - strand, within MMP_RS07720 at 1462.609 kb on + strand, within MMP_RS07720 at 1462.624 kb on - strand, within MMP_RS07720 at 1462.644 kb on + strand, within MMP_RS07720 at 1462.644 kb on + strand, within MMP_RS07720 at 1462.739 kb on - strand, within MMP_RS07720 at 1462.740 kb on + strand, within MMP_RS07720 at 1462.740 kb on + strand, within MMP_RS07720 at 1462.740 kb on + strand, within MMP_RS07720 at 1462.740 kb on + strand, within MMP_RS07720 at 1462.769 kb on - strand, within MMP_RS07720 at 1462.769 kb on - strand, within MMP_RS07720 at 1462.772 kb on - strand, within MMP_RS07720 at 1462.775 kb on - strand, within MMP_RS07720 at 1462.775 kb on - strand, within MMP_RS07720 at 1462.814 kb on - strand, within MMP_RS07720 at 1462.814 kb on - strand, within MMP_RS07720 at 1462.814 kb on - strand, within MMP_RS07720 at 1462.815 kb on + strand, within MMP_RS07720 at 1462.853 kb on + strand, within MMP_RS07720 at 1462.888 kb on - strand, within MMP_RS07720 at 1462.888 kb on - strand, within MMP_RS07720 at 1462.889 kb on + strand, within MMP_RS07720 at 1462.933 kb on - strand at 1462.943 kb on - strand at 1462.944 kb on + strand at 1462.979 kb on + strand at 1462.996 kb on - strand at 1463.202 kb on - strand, within MMP_RS07725 at 1463.203 kb on + strand, within MMP_RS07725 at 1463.317 kb on - strand, within MMP_RS07725 at 1463.332 kb on - strand, within MMP_RS07725 at 1463.740 kb on + strand, within MMP_RS07730 at 1463.848 kb on - strand, within MMP_RS07730 at 1463.892 kb on - strand, within MMP_RS07730 at 1463.893 kb on + strand, within MMP_RS07730 at 1464.006 kb on - strand, within MMP_RS07730 at 1464.393 kb on - strand at 1464.518 kb on - strand at 1464.773 kb on - strand, within MMP_RS07735 at 1464.993 kb on - strand, within MMP_RS07735
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 1,462,559 + +0.9 1,462,597 - MMP_RS07720 0.15 +0.5 1,462,605 - MMP_RS07720 0.17 +1.5 1,462,609 + MMP_RS07720 0.18 +0.5 1,462,624 - MMP_RS07720 0.21 +0.2 1,462,644 + MMP_RS07720 0.26 +0.2 1,462,644 + MMP_RS07720 0.26 +0.6 1,462,739 - MMP_RS07720 0.49 -0.1 1,462,740 + MMP_RS07720 0.49 +0.1 1,462,740 + MMP_RS07720 0.49 +0.5 1,462,740 + MMP_RS07720 0.49 +0.3 1,462,740 + MMP_RS07720 0.49 -0.3 1,462,769 - MMP_RS07720 0.56 +0.2 1,462,769 - MMP_RS07720 0.56 +0.1 1,462,772 - MMP_RS07720 0.57 +0.4 1,462,775 - MMP_RS07720 0.58 +0.1 1,462,775 - MMP_RS07720 0.58 -0.7 1,462,814 - MMP_RS07720 0.67 -0.1 1,462,814 - MMP_RS07720 0.67 -0.3 1,462,814 - MMP_RS07720 0.67 +1.2 1,462,815 + MMP_RS07720 0.67 +0.3 1,462,853 + MMP_RS07720 0.76 +0.1 1,462,888 - MMP_RS07720 0.85 -0.4 1,462,888 - MMP_RS07720 0.85 +0.7 1,462,889 + MMP_RS07720 0.85 +0.3 1,462,933 - +0.2 1,462,943 - -0.7 1,462,944 + +0.3 1,462,979 + -0.5 1,462,996 - +0.4 1,463,202 - MMP_RS07725 0.46 +0.4 1,463,203 + MMP_RS07725 0.46 +0.4 1,463,317 - MMP_RS07725 0.69 +0.5 1,463,332 - MMP_RS07725 0.72 +0.4 1,463,740 + MMP_RS07730 0.27 -1.1 1,463,848 - MMP_RS07730 0.39 -4.0 1,463,892 - MMP_RS07730 0.44 -1.5 1,463,893 + MMP_RS07730 0.44 -2.7 1,464,006 - MMP_RS07730 0.57 -3.6 1,464,393 - -1.8 1,464,518 - -3.3 1,464,773 - MMP_RS07735 0.31 -3.3 1,464,993 - MMP_RS07735 0.50 -1.8
Or see this region's nucleotide sequence