Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06920 and MMP_RS06925 are separated by 8 nucleotides MMP_RS06925 and MMP_RS06930 are separated by 16 nucleotides MMP_RS06930 and MMP_RS06935 are separated by 9 nucleotides
MMP_RS06920: MMP_RS06920 - AAA family ATPase, at 1,321,087 to 1,324,068
_RS06920
MMP_RS06925: MMP_RS06925 - ribonuclease VapC4, at 1,324,077 to 1,324,472
_RS06925
MMP_RS06930: MMP_RS06930 - MBL fold metallo-hydrolase, at 1,324,489 to 1,325,289
_RS06930
MMP_RS06935: MMP_RS06935 - protease complex subunit PrcB family protein, at 1,325,299 to 1,325,775
_RS06935
Position (kb)
1324
1325 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1323.117 kb on + strand, within MMP_RS06920 at 1323.178 kb on - strand, within MMP_RS06920 at 1323.231 kb on - strand, within MMP_RS06920 at 1323.416 kb on + strand, within MMP_RS06920 at 1323.536 kb on - strand, within MMP_RS06920 at 1323.703 kb on + strand, within MMP_RS06920 at 1323.836 kb on - strand at 1324.023 kb on + strand at 1324.056 kb on + strand at 1324.087 kb on - strand at 1324.154 kb on - strand, within MMP_RS06925 at 1324.161 kb on + strand, within MMP_RS06925 at 1324.181 kb on + strand, within MMP_RS06925 at 1324.184 kb on - strand, within MMP_RS06925 at 1324.364 kb on + strand, within MMP_RS06925 at 1324.461 kb on - strand at 1324.466 kb on + strand at 1324.497 kb on - strand at 1324.521 kb on - strand at 1324.587 kb on - strand, within MMP_RS06930 at 1324.588 kb on + strand, within MMP_RS06930 at 1324.590 kb on + strand, within MMP_RS06930 at 1324.590 kb on + strand, within MMP_RS06930 at 1324.634 kb on - strand, within MMP_RS06930 at 1324.675 kb on - strand, within MMP_RS06930 at 1324.684 kb on - strand, within MMP_RS06930 at 1324.685 kb on + strand, within MMP_RS06930 at 1324.777 kb on - strand, within MMP_RS06930 at 1324.924 kb on - strand, within MMP_RS06930 at 1324.925 kb on + strand, within MMP_RS06930 at 1325.080 kb on + strand, within MMP_RS06930 at 1325.081 kb on - strand, within MMP_RS06930 at 1325.082 kb on + strand, within MMP_RS06930 at 1325.082 kb on + strand, within MMP_RS06930 at 1325.082 kb on + strand, within MMP_RS06930 at 1325.103 kb on - strand, within MMP_RS06930 at 1325.103 kb on - strand, within MMP_RS06930 at 1325.107 kb on + strand, within MMP_RS06930 at 1325.107 kb on + strand, within MMP_RS06930 at 1325.107 kb on + strand, within MMP_RS06930 at 1325.110 kb on - strand, within MMP_RS06930 at 1325.141 kb on + strand, within MMP_RS06930 at 1325.241 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 1,323,117 + MMP_RS06920 0.68 -0.3 1,323,178 - MMP_RS06920 0.70 +0.3 1,323,231 - MMP_RS06920 0.72 -0.1 1,323,416 + MMP_RS06920 0.78 +0.2 1,323,536 - MMP_RS06920 0.82 +0.1 1,323,703 + MMP_RS06920 0.88 -0.4 1,323,836 - +0.4 1,324,023 + -0.3 1,324,056 + -0.2 1,324,087 - -3.0 1,324,154 - MMP_RS06925 0.19 -4.4 1,324,161 + MMP_RS06925 0.21 -1.6 1,324,181 + MMP_RS06925 0.26 -2.6 1,324,184 - MMP_RS06925 0.27 -3.5 1,324,364 + MMP_RS06925 0.72 -4.2 1,324,461 - +0.0 1,324,466 + +0.4 1,324,497 - -0.0 1,324,521 - +0.6 1,324,587 - MMP_RS06930 0.12 +0.7 1,324,588 + MMP_RS06930 0.12 +0.0 1,324,590 + MMP_RS06930 0.13 +0.3 1,324,590 + MMP_RS06930 0.13 +0.1 1,324,634 - MMP_RS06930 0.18 +0.5 1,324,675 - MMP_RS06930 0.23 +0.8 1,324,684 - MMP_RS06930 0.24 +0.3 1,324,685 + MMP_RS06930 0.24 +0.7 1,324,777 - MMP_RS06930 0.36 +0.1 1,324,924 - MMP_RS06930 0.54 +0.8 1,324,925 + MMP_RS06930 0.54 +0.8 1,325,080 + MMP_RS06930 0.74 -0.3 1,325,081 - MMP_RS06930 0.74 +0.7 1,325,082 + MMP_RS06930 0.74 +0.0 1,325,082 + MMP_RS06930 0.74 -0.7 1,325,082 + MMP_RS06930 0.74 +0.4 1,325,103 - MMP_RS06930 0.77 -0.1 1,325,103 - MMP_RS06930 0.77 +0.3 1,325,107 + MMP_RS06930 0.77 +0.6 1,325,107 + MMP_RS06930 0.77 +0.2 1,325,107 + MMP_RS06930 0.77 +0.4 1,325,110 - MMP_RS06930 0.78 +1.1 1,325,141 + MMP_RS06930 0.81 +0.3 1,325,241 - +0.4
Or see this region's nucleotide sequence