Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06400

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06390 and MMP_RS06395 are separated by 52 nucleotidesMMP_RS06395 and MMP_RS06400 are separated by 203 nucleotidesMMP_RS06400 and MMP_RS06405 are separated by 23 nucleotides MMP_RS06390: MMP_RS06390 - quinolinate synthase NadA, at 1,227,185 to 1,228,102 _RS06390 MMP_RS06395: MMP_RS06395 - ThiF family adenylyltransferase, at 1,228,155 to 1,228,769 _RS06395 MMP_RS06400: MMP_RS06400 - 4Fe-4S binding protein, at 1,228,973 to 1,229,419 _RS06400 MMP_RS06405: MMP_RS06405 - 4Fe-4S binding protein, at 1,229,443 to 1,230,501 _RS06405 Position (kb) 1228 1229 1230Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1228.036 kb on + strandat 1228.094 kb on - strandat 1228.094 kb on - strandat 1228.416 kb on - strand, within MMP_RS06395at 1228.509 kb on + strand, within MMP_RS06395at 1228.568 kb on + strand, within MMP_RS06395at 1228.603 kb on + strand, within MMP_RS06395at 1228.798 kb on + strandat 1228.837 kb on + strandat 1228.928 kb on - strandat 1228.929 kb on + strandat 1228.929 kb on + strandat 1228.929 kb on + strandat 1228.929 kb on + strandat 1228.951 kb on + strandat 1228.985 kb on + strandat 1228.985 kb on + strandat 1228.985 kb on + strandat 1228.986 kb on - strandat 1228.986 kb on - strandat 1228.987 kb on + strandat 1228.987 kb on + strandat 1228.990 kb on + strandat 1229.234 kb on - strand, within MMP_RS06400at 1229.242 kb on + strand, within MMP_RS06400at 1229.251 kb on - strand, within MMP_RS06400at 1229.439 kb on - strandat 1229.440 kb on + strandat 1230.027 kb on + strandat 1230.083 kb on + strandat 1230.357 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
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1,228,036 + -3.7
1,228,094 - -2.2
1,228,094 - -2.4
1,228,416 - MMP_RS06395 0.42 -1.7
1,228,509 + MMP_RS06395 0.58 -2.9
1,228,568 + MMP_RS06395 0.67 -2.3
1,228,603 + MMP_RS06395 0.73 -2.2
1,228,798 + -0.5
1,228,837 + -0.5
1,228,928 - -3.6
1,228,929 + -3.8
1,228,929 + -4.1
1,228,929 + -4.9
1,228,929 + -4.4
1,228,951 + -4.1
1,228,985 + -3.1
1,228,985 + -4.4
1,228,985 + -4.0
1,228,986 - -3.2
1,228,986 - -3.9
1,228,987 + -4.5
1,228,987 + -5.0
1,228,990 + -2.9
1,229,234 - MMP_RS06400 0.58 -3.0
1,229,242 + MMP_RS06400 0.60 -3.4
1,229,251 - MMP_RS06400 0.62 -4.0
1,229,439 - -1.4
1,229,440 + -3.5
1,230,027 + -3.4
1,230,083 + -2.9
1,230,357 + -2.2

Or see this region's nucleotide sequence