Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02185 and MMP_RS02190 are separated by 70 nucleotides MMP_RS02190 and MMP_RS02195 are separated by 34 nucleotides MMP_RS02195 and MMP_RS02200 are separated by 150 nucleotides
MMP_RS02185: MMP_RS02185 - ribose-phosphate diphosphokinase, at 408,933 to 409,796
_RS02185
MMP_RS02190: MMP_RS02190 - sulfopyruvate decarboxylase subunit alpha, at 409,867 to 410,370
_RS02190
MMP_RS02195: MMP_RS02195 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase, at 410,405 to 411,682
_RS02195
MMP_RS02200: MMP_RS02200 - methyl-accepting chemotaxis protein, at 411,833 to 414,028
_RS02200
Position (kb) 410
411
412 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 409.769 kb on + strand at 409.798 kb on - strand at 409.798 kb on - strand at 409.903 kb on - strand at 409.930 kb on + strand, within MMP_RS02190 at 410.106 kb on - strand, within MMP_RS02190 at 410.274 kb on + strand, within MMP_RS02190 at 410.349 kb on + strand at 410.371 kb on + strand at 410.527 kb on + strand at 410.622 kb on - strand, within MMP_RS02195 at 410.845 kb on - strand, within MMP_RS02195 at 410.872 kb on - strand, within MMP_RS02195 at 410.884 kb on + strand, within MMP_RS02195 at 411.366 kb on - strand, within MMP_RS02195 at 411.484 kb on + strand, within MMP_RS02195 at 411.540 kb on - strand, within MMP_RS02195 at 411.560 kb on + strand at 411.580 kb on - strand at 411.581 kb on + strand at 411.710 kb on - strand at 411.743 kb on - strand at 411.743 kb on - strand at 411.744 kb on + strand at 411.782 kb on - strand at 411.783 kb on + strand at 411.784 kb on - strand at 411.784 kb on - strand at 411.784 kb on - strand at 411.784 kb on - strand at 411.784 kb on - strand at 411.785 kb on + strand at 411.785 kb on + strand at 411.786 kb on - strand at 411.786 kb on - strand at 411.786 kb on - strand at 411.786 kb on - strand at 411.786 kb on - strand at 411.786 kb on - strand at 411.787 kb on + strand at 411.787 kb on + strand at 411.787 kb on + strand at 411.787 kb on + strand at 411.806 kb on + strand at 411.817 kb on - strand at 411.818 kb on + strand at 411.819 kb on - strand at 411.819 kb on - strand at 411.819 kb on - strand at 411.895 kb on + strand at 411.928 kb on - strand at 411.931 kb on - strand at 411.938 kb on + strand at 412.053 kb on + strand, within MMP_RS02200 at 412.093 kb on + strand, within MMP_RS02200 at 412.201 kb on - strand, within MMP_RS02200 at 412.202 kb on + strand, within MMP_RS02200 at 412.291 kb on - strand, within MMP_RS02200 at 412.349 kb on + strand, within MMP_RS02200 at 412.540 kb on + strand, within MMP_RS02200 at 412.616 kb on - strand, within MMP_RS02200 at 412.617 kb on + strand, within MMP_RS02200 at 412.617 kb on + strand, within MMP_RS02200 at 412.645 kb on - strand, within MMP_RS02200
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 409,769 + -2.0 409,798 - -3.0 409,798 - -4.7 409,903 - -3.3 409,930 + MMP_RS02190 0.12 -4.8 410,106 - MMP_RS02190 0.47 -3.4 410,274 + MMP_RS02190 0.81 -4.3 410,349 + -2.9 410,371 + -3.1 410,527 + -3.7 410,622 - MMP_RS02195 0.17 -4.8 410,845 - MMP_RS02195 0.34 -4.4 410,872 - MMP_RS02195 0.37 -4.4 410,884 + MMP_RS02195 0.37 -4.3 411,366 - MMP_RS02195 0.75 -5.4 411,484 + MMP_RS02195 0.84 -3.5 411,540 - MMP_RS02195 0.89 -2.4 411,560 + -2.8 411,580 - -0.5 411,581 + -3.8 411,710 - +0.6 411,743 - +0.4 411,743 - +0.5 411,744 + +0.5 411,782 - +0.8 411,783 + +0.1 411,784 - +0.4 411,784 - +0.2 411,784 - +0.1 411,784 - -0.0 411,784 - -0.1 411,785 + +0.5 411,785 + +0.2 411,786 - -0.0 411,786 - -0.2 411,786 - -0.4 411,786 - -0.2 411,786 - -0.8 411,786 - +0.1 411,787 + -0.0 411,787 + -0.3 411,787 + -0.9 411,787 + +0.5 411,806 + -0.3 411,817 - -0.8 411,818 + -0.1 411,819 - -0.1 411,819 - +0.1 411,819 - +0.5 411,895 + -0.5 411,928 - +0.1 411,931 - -1.1 411,938 + -0.3 412,053 + MMP_RS02200 0.10 -0.1 412,093 + MMP_RS02200 0.12 -0.2 412,201 - MMP_RS02200 0.17 +0.1 412,202 + MMP_RS02200 0.17 +0.0 412,291 - MMP_RS02200 0.21 -0.4 412,349 + MMP_RS02200 0.23 +0.1 412,540 + MMP_RS02200 0.32 +0.0 412,616 - MMP_RS02200 0.36 -0.1 412,617 + MMP_RS02200 0.36 +0.2 412,617 + MMP_RS02200 0.36 -0.7 412,645 - MMP_RS02200 0.37 -0.1
Or see this region's nucleotide sequence