Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01160 and MMP_RS01165 are separated by 95 nucleotides MMP_RS01165 and MMP_RS01170 are separated by 1 nucleotides MMP_RS01170 and MMP_RS01175 are separated by 11 nucleotides MMP_RS01175 and MMP_RS01180 are separated by 11 nucleotides
MMP_RS01160: MMP_RS01160 - DMT family transporter, at 217,251 to 218,150
_RS01160
MMP_RS01165: MMP_RS01165 - DUF434 domain-containing protein, at 218,246 to 218,914
_RS01165
MMP_RS01170: MMP_RS01170 - glycine--tRNA ligase, at 218,916 to 220,640
_RS01170
MMP_RS01175: MMP_RS01175 - triphosphoribosyl-dephospho-CoA synthase, at 220,652 to 221,533
_RS01175
MMP_RS01180: MMP_RS01180 - XTP/dITP diphosphatase, at 221,545 to 222,096
_RS01180
Position (kb) 218
219
220
221 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 217.929 kb on - strand, within MMP_RS01160 at 217.930 kb on + strand, within MMP_RS01160 at 217.930 kb on + strand, within MMP_RS01160 at 217.930 kb on + strand, within MMP_RS01160 at 217.930 kb on + strand, within MMP_RS01160 at 218.121 kb on - strand at 218.122 kb on + strand at 218.456 kb on + strand, within MMP_RS01165 at 218.734 kb on - strand, within MMP_RS01165 at 218.764 kb on - strand, within MMP_RS01165 at 218.806 kb on - strand, within MMP_RS01165 at 218.806 kb on - strand, within MMP_RS01165 at 218.830 kb on - strand, within MMP_RS01165 at 218.858 kb on + strand at 219.809 kb on + strand at 219.924 kb on - strand at 220.486 kb on - strand at 220.895 kb on - strand, within MMP_RS01175 at 220.948 kb on + strand, within MMP_RS01175 at 220.951 kb on + strand, within MMP_RS01175 at 220.977 kb on - strand, within MMP_RS01175 at 221.236 kb on - strand, within MMP_RS01175 at 221.241 kb on + strand, within MMP_RS01175 at 221.568 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 217,929 - MMP_RS01160 0.75 +0.1 217,930 + MMP_RS01160 0.75 +0.4 217,930 + MMP_RS01160 0.75 -0.4 217,930 + MMP_RS01160 0.75 +0.2 217,930 + MMP_RS01160 0.75 +1.1 218,121 - +0.5 218,122 + -0.1 218,456 + MMP_RS01165 0.31 -0.2 218,734 - MMP_RS01165 0.73 -0.0 218,764 - MMP_RS01165 0.77 -0.6 218,806 - MMP_RS01165 0.84 +0.4 218,806 - MMP_RS01165 0.84 +1.2 218,830 - MMP_RS01165 0.87 -0.1 218,858 + +0.9 219,809 + -2.3 219,924 - -3.3 220,486 - -1.5 220,895 - MMP_RS01175 0.28 -5.6 220,948 + MMP_RS01175 0.34 -6.7 220,951 + MMP_RS01175 0.34 -5.2 220,977 - MMP_RS01175 0.37 -5.3 221,236 - MMP_RS01175 0.66 -5.3 221,241 + MMP_RS01175 0.67 -5.3 221,568 - +0.1
Or see this region's nucleotide sequence