Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01030 and MMP_RS01035 are separated by 14 nucleotides MMP_RS01035 and MMP_RS01040 are separated by 69 nucleotides MMP_RS01040 and MMP_RS01045 are separated by 89 nucleotides
MMP_RS01030: MMP_RS01030 - MTAP family purine nucleoside phosphorylase, at 193,763 to 194,524
_RS01030
MMP_RS01035: MMP_RS01035 - threonylcarbamoyl-AMP synthase, at 194,539 to 195,159
_RS01035
MMP_RS01040: MMP_RS01040 - phosphomethylpyrimidine synthase, at 195,229 to 196,509
_RS01040
MMP_RS01045: MMP_RS01045 - diphthamide biosynthesis enzyme Dph2, at 196,599 to 197,528
_RS01045
Position (kb) 195
196
197 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 194.407 kb on + strand, within MMP_RS01030 at 194.458 kb on - strand at 194.918 kb on - strand at 195.337 kb on + strand at 196.329 kb on - strand at 196.330 kb on + strand at 196.540 kb on - strand at 196.542 kb on - strand at 196.554 kb on - strand at 196.558 kb on + strand at 196.560 kb on - strand at 196.560 kb on - strand at 196.560 kb on - strand at 196.564 kb on - strand at 196.564 kb on - strand at 196.565 kb on + strand at 196.565 kb on + strand at 196.599 kb on + strand at 196.781 kb on + strand, within MMP_RS01045 at 196.817 kb on + strand, within MMP_RS01045 at 196.892 kb on - strand, within MMP_RS01045 at 196.959 kb on + strand, within MMP_RS01045 at 196.998 kb on + strand, within MMP_RS01045 at 196.998 kb on + strand, within MMP_RS01045 at 197.096 kb on - strand, within MMP_RS01045 at 197.209 kb on - strand, within MMP_RS01045 at 197.209 kb on - strand, within MMP_RS01045 at 197.322 kb on - strand, within MMP_RS01045 at 197.323 kb on + strand, within MMP_RS01045 at 197.325 kb on + strand, within MMP_RS01045 at 197.443 kb on - strand at 197.443 kb on - strand at 197.470 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 194,407 + MMP_RS01030 0.85 -0.1 194,458 - -3.5 194,918 - -2.9 195,337 + -3.3 196,329 - -4.2 196,330 + -2.6 196,540 - -6.2 196,542 - -2.9 196,554 - -2.5 196,558 + -2.7 196,560 - -2.6 196,560 - -2.3 196,560 - -1.7 196,564 - -1.8 196,564 - -1.2 196,565 + -1.7 196,565 + -2.3 196,599 + -1.9 196,781 + MMP_RS01045 0.20 -1.8 196,817 + MMP_RS01045 0.23 -1.6 196,892 - MMP_RS01045 0.32 -1.9 196,959 + MMP_RS01045 0.39 -2.6 196,998 + MMP_RS01045 0.43 -1.8 196,998 + MMP_RS01045 0.43 -1.9 197,096 - MMP_RS01045 0.53 -1.4 197,209 - MMP_RS01045 0.66 -1.9 197,209 - MMP_RS01045 0.66 -2.0 197,322 - MMP_RS01045 0.78 -2.1 197,323 + MMP_RS01045 0.78 -0.5 197,325 + MMP_RS01045 0.78 -1.2 197,443 - -3.2 197,443 - -2.1 197,470 + -2.5
Or see this region's nucleotide sequence