Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_901 and Ac3H11_902 are separated by 134 nucleotides Ac3H11_902 and Ac3H11_903 are separated by 90 nucleotides
Ac3H11_901 - Beta-lactamase (EC 3.5.2.6), at 12,819 to 13,700
_901
Ac3H11_902 - Glycine cleavage system transcriptional activator, at 13,835 to 14,713
_902
Ac3H11_903 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 14,804 to 17,833
_903
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 12.907 kb on + strand at 13.387 kb on + strand, within Ac3H11_901 at 13.387 kb on + strand, within Ac3H11_901 at 13.722 kb on - strand at 13.760 kb on + strand at 13.792 kb on + strand at 13.792 kb on + strand at 13.792 kb on + strand at 14.784 kb on + strand at 14.805 kb on + strand at 14.805 kb on + strand at 14.805 kb on + strand at 14.805 kb on + strand at 14.805 kb on + strand at 14.805 kb on + strand at 14.806 kb on - strand at 14.806 kb on - strand at 14.806 kb on - strand at 15.064 kb on - strand at 15.240 kb on + strand, within Ac3H11_903 at 15.240 kb on + strand, within Ac3H11_903 at 15.240 kb on + strand, within Ac3H11_903 at 15.240 kb on + strand, within Ac3H11_903 at 15.240 kb on + strand, within Ac3H11_903 at 15.241 kb on - strand, within Ac3H11_903 at 15.241 kb on - strand, within Ac3H11_903 at 15.241 kb on - strand, within Ac3H11_903 at 15.281 kb on - strand, within Ac3H11_903 at 15.281 kb on - strand, within Ac3H11_903 at 15.486 kb on + strand, within Ac3H11_903 at 15.486 kb on + strand, within Ac3H11_903 at 15.486 kb on + strand, within Ac3H11_903 at 15.486 kb on + strand, within Ac3H11_903 at 15.499 kb on - strand, within Ac3H11_903 at 15.499 kb on - strand, within Ac3H11_903 at 15.538 kb on + strand, within Ac3H11_903 at 15.539 kb on - strand, within Ac3H11_903 at 15.539 kb on - strand, within Ac3H11_903 at 15.636 kb on + strand, within Ac3H11_903 at 15.636 kb on + strand, within Ac3H11_903
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 12,907 + -0.5 13,387 + Ac3H11_901 0.64 +1.5 13,387 + Ac3H11_901 0.64 +1.4 13,722 - -0.7 13,760 + -1.6 13,792 + +1.6 13,792 + -0.6 13,792 + +2.5 14,784 + +0.7 14,805 + -0.9 14,805 + +0.1 14,805 + -0.2 14,805 + +0.2 14,805 + +0.6 14,805 + +1.0 14,806 - +0.1 14,806 - -0.5 14,806 - +0.5 15,064 - -1.1 15,240 + Ac3H11_903 0.14 +0.1 15,240 + Ac3H11_903 0.14 -0.2 15,240 + Ac3H11_903 0.14 -1.7 15,240 + Ac3H11_903 0.14 -1.0 15,240 + Ac3H11_903 0.14 -0.6 15,241 - Ac3H11_903 0.14 +0.3 15,241 - Ac3H11_903 0.14 -0.2 15,241 - Ac3H11_903 0.14 -1.1 15,281 - Ac3H11_903 0.16 -1.0 15,281 - Ac3H11_903 0.16 +0.0 15,486 + Ac3H11_903 0.23 -0.1 15,486 + Ac3H11_903 0.23 +1.1 15,486 + Ac3H11_903 0.23 +1.0 15,486 + Ac3H11_903 0.23 +0.6 15,499 - Ac3H11_903 0.23 -1.0 15,499 - Ac3H11_903 0.23 -0.9 15,538 + Ac3H11_903 0.24 -0.2 15,539 - Ac3H11_903 0.24 -2.7 15,539 - Ac3H11_903 0.24 +0.3 15,636 + Ac3H11_903 0.27 +0.8 15,636 + Ac3H11_903 0.27 -1.1
Or see this region's nucleotide sequence