Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4982 and Ac3H11_4983 are separated by 156 nucleotides Ac3H11_4983 and Ac3H11_4984 overlap by 4 nucleotides Ac3H11_4984 and Ac3H11_4985 are separated by 10 nucleotides
Ac3H11_4982 - Transcriptional regulator, TetR family, at 146,113 to 146,970
_4982
Ac3H11_4983 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 147,127 to 147,969
_4983
Ac3H11_4984 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 147,966 to 148,856
_4984
Ac3H11_4985 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 148,867 to 149,904
_4985
Position (kb) 147
148
149 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 146.993 kb on + strand at 147.114 kb on + strand at 147.115 kb on - strand at 147.117 kb on + strand at 147.117 kb on + strand at 147.117 kb on + strand at 147.117 kb on + strand at 147.118 kb on - strand at 147.224 kb on + strand, within Ac3H11_4983 at 147.225 kb on - strand, within Ac3H11_4983 at 147.317 kb on - strand, within Ac3H11_4983 at 147.622 kb on + strand, within Ac3H11_4983 at 147.990 kb on + strand at 147.990 kb on + strand at 147.990 kb on + strand at 147.991 kb on - strand at 147.991 kb on - strand at 148.036 kb on - strand at 148.069 kb on - strand, within Ac3H11_4984 at 148.069 kb on - strand, within Ac3H11_4984 at 148.069 kb on - strand, within Ac3H11_4984 at 148.125 kb on + strand, within Ac3H11_4984 at 148.125 kb on + strand, within Ac3H11_4984 at 148.125 kb on + strand, within Ac3H11_4984 at 148.126 kb on - strand, within Ac3H11_4984 at 148.126 kb on - strand, within Ac3H11_4984 at 148.126 kb on - strand, within Ac3H11_4984 at 148.518 kb on + strand, within Ac3H11_4984 at 148.519 kb on - strand, within Ac3H11_4984 at 148.750 kb on - strand, within Ac3H11_4984 at 148.764 kb on + strand, within Ac3H11_4984 at 148.765 kb on - strand, within Ac3H11_4984 at 148.765 kb on - strand, within Ac3H11_4984 at 148.818 kb on + strand at 148.854 kb on + strand at 148.894 kb on + strand at 148.894 kb on + strand at 148.895 kb on - strand at 148.895 kb on - strand at 149.003 kb on - strand, within Ac3H11_4985 at 149.116 kb on + strand, within Ac3H11_4985 at 149.117 kb on - strand, within Ac3H11_4985 at 149.117 kb on - strand, within Ac3H11_4985 at 149.117 kb on - strand, within Ac3H11_4985 at 149.242 kb on + strand, within Ac3H11_4985 at 149.243 kb on - strand, within Ac3H11_4985 at 149.243 kb on - strand, within Ac3H11_4985 at 149.243 kb on - strand, within Ac3H11_4985 at 149.383 kb on + strand, within Ac3H11_4985 at 149.506 kb on - strand, within Ac3H11_4985 at 149.507 kb on + strand, within Ac3H11_4985 at 149.507 kb on + strand, within Ac3H11_4985 at 149.507 kb on + strand, within Ac3H11_4985 at 149.508 kb on - strand, within Ac3H11_4985 at 149.521 kb on + strand, within Ac3H11_4985 at 149.521 kb on + strand, within Ac3H11_4985 at 149.555 kb on - strand, within Ac3H11_4985 at 149.555 kb on - strand, within Ac3H11_4985 at 149.555 kb on - strand, within Ac3H11_4985 at 149.584 kb on + strand, within Ac3H11_4985 at 149.585 kb on - strand, within Ac3H11_4985 at 149.798 kb on + strand, within Ac3H11_4985 at 149.798 kb on + strand, within Ac3H11_4985 at 149.799 kb on - strand, within Ac3H11_4985 at 149.799 kb on - strand, within Ac3H11_4985 at 149.799 kb on - strand, within Ac3H11_4985
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 146,993 + -1.2 147,114 + +1.7 147,115 - -1.1 147,117 + -0.5 147,117 + -0.2 147,117 + +1.4 147,117 + -0.1 147,118 - -1.3 147,224 + Ac3H11_4983 0.12 +0.6 147,225 - Ac3H11_4983 0.12 +0.6 147,317 - Ac3H11_4983 0.23 +2.6 147,622 + Ac3H11_4983 0.59 +0.2 147,990 + +1.0 147,990 + +0.2 147,990 + -1.1 147,991 - +1.2 147,991 - +0.9 148,036 - +0.9 148,069 - Ac3H11_4984 0.12 +1.3 148,069 - Ac3H11_4984 0.12 -0.7 148,069 - Ac3H11_4984 0.12 +0.3 148,125 + Ac3H11_4984 0.18 +0.1 148,125 + Ac3H11_4984 0.18 +0.4 148,125 + Ac3H11_4984 0.18 -1.4 148,126 - Ac3H11_4984 0.18 +1.7 148,126 - Ac3H11_4984 0.18 -2.1 148,126 - Ac3H11_4984 0.18 +2.4 148,518 + Ac3H11_4984 0.62 -0.4 148,519 - Ac3H11_4984 0.62 -0.6 148,750 - Ac3H11_4984 0.88 -0.6 148,764 + Ac3H11_4984 0.90 +0.4 148,765 - Ac3H11_4984 0.90 +1.4 148,765 - Ac3H11_4984 0.90 +0.4 148,818 + -0.2 148,854 + +0.5 148,894 + +0.3 148,894 + +0.4 148,895 - +0.1 148,895 - -0.9 149,003 - Ac3H11_4985 0.13 -0.1 149,116 + Ac3H11_4985 0.24 -0.1 149,117 - Ac3H11_4985 0.24 +2.6 149,117 - Ac3H11_4985 0.24 -0.0 149,117 - Ac3H11_4985 0.24 -0.2 149,242 + Ac3H11_4985 0.36 +0.8 149,243 - Ac3H11_4985 0.36 -2.3 149,243 - Ac3H11_4985 0.36 +1.2 149,243 - Ac3H11_4985 0.36 +0.6 149,383 + Ac3H11_4985 0.50 -0.5 149,506 - Ac3H11_4985 0.62 +2.9 149,507 + Ac3H11_4985 0.62 -0.6 149,507 + Ac3H11_4985 0.62 +1.8 149,507 + Ac3H11_4985 0.62 -1.7 149,508 - Ac3H11_4985 0.62 -2.5 149,521 + Ac3H11_4985 0.63 -1.3 149,521 + Ac3H11_4985 0.63 -0.2 149,555 - Ac3H11_4985 0.66 +0.2 149,555 - Ac3H11_4985 0.66 +1.1 149,555 - Ac3H11_4985 0.66 +0.1 149,584 + Ac3H11_4985 0.69 -0.3 149,585 - Ac3H11_4985 0.69 +3.0 149,798 + Ac3H11_4985 0.90 -1.5 149,798 + Ac3H11_4985 0.90 +0.7 149,799 - Ac3H11_4985 0.90 -0.1 149,799 - Ac3H11_4985 0.90 +1.6 149,799 - Ac3H11_4985 0.90 +0.9
Or see this region's nucleotide sequence