Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1345 and Ac3H11_1346 are separated by 163 nucleotides Ac3H11_1346 and Ac3H11_1347 are separated by 178 nucleotides Ac3H11_1347 and Ac3H11_1348 are separated by 48 nucleotides
Ac3H11_1345 - putative exported protein, at 35,072 to 38,914
_1345
Ac3H11_1346 - Ser-tRNA(Ala) deacylase; Gly-tRNA(Ala) deacylase, at 39,078 to 39,830
_1346
Ac3H11_1347 - hypothetical protein, at 40,009 to 40,308
_1347
Ac3H11_1348 - Creatinine amidohydrolase (EC 3.5.2.10), at 40,357 to 41,220
_1348
Position (kb) 39
40 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 38.160 kb on + strand, within Ac3H11_1345 at 38.160 kb on + strand, within Ac3H11_1345 at 38.161 kb on - strand, within Ac3H11_1345 at 38.161 kb on - strand, within Ac3H11_1345 at 38.161 kb on - strand, within Ac3H11_1345 at 38.169 kb on + strand, within Ac3H11_1345 at 38.169 kb on + strand, within Ac3H11_1345 at 38.169 kb on + strand, within Ac3H11_1345 at 38.170 kb on - strand, within Ac3H11_1345 at 38.170 kb on - strand, within Ac3H11_1345 at 38.170 kb on - strand, within Ac3H11_1345 at 38.170 kb on - strand, within Ac3H11_1345 at 38.170 kb on - strand, within Ac3H11_1345 at 38.424 kb on + strand, within Ac3H11_1345 at 38.424 kb on + strand, within Ac3H11_1345 at 38.424 kb on + strand, within Ac3H11_1345 at 38.425 kb on - strand, within Ac3H11_1345 at 38.425 kb on - strand, within Ac3H11_1345 at 38.425 kb on - strand, within Ac3H11_1345 at 38.508 kb on + strand, within Ac3H11_1345 at 38.509 kb on - strand, within Ac3H11_1345 at 38.613 kb on + strand at 38.613 kb on + strand at 38.614 kb on - strand at 38.614 kb on - strand at 38.614 kb on - strand at 38.614 kb on - strand at 38.614 kb on - strand at 38.977 kb on + strand at 39.727 kb on + strand, within Ac3H11_1346 at 39.800 kb on - strand at 39.881 kb on + strand at 39.882 kb on - strand at 39.882 kb on - strand at 39.882 kb on - strand at 39.939 kb on + strand at 39.940 kb on - strand at 39.945 kb on + strand at 39.945 kb on + strand at 39.946 kb on - strand at 40.072 kb on + strand, within Ac3H11_1347 at 40.072 kb on + strand, within Ac3H11_1347 at 40.072 kb on + strand, within Ac3H11_1347 at 40.073 kb on - strand, within Ac3H11_1347 at 40.073 kb on - strand, within Ac3H11_1347 at 40.073 kb on - strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.088 kb on - strand, within Ac3H11_1347
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 38,160 + Ac3H11_1345 0.80 -0.3 38,160 + Ac3H11_1345 0.80 +0.8 38,161 - Ac3H11_1345 0.80 -0.3 38,161 - Ac3H11_1345 0.80 +1.2 38,161 - Ac3H11_1345 0.80 -1.4 38,169 + Ac3H11_1345 0.81 +0.2 38,169 + Ac3H11_1345 0.81 +0.3 38,169 + Ac3H11_1345 0.81 +0.9 38,170 - Ac3H11_1345 0.81 -0.9 38,170 - Ac3H11_1345 0.81 -1.1 38,170 - Ac3H11_1345 0.81 +0.1 38,170 - Ac3H11_1345 0.81 -0.3 38,170 - Ac3H11_1345 0.81 +0.0 38,424 + Ac3H11_1345 0.87 +1.0 38,424 + Ac3H11_1345 0.87 -0.8 38,424 + Ac3H11_1345 0.87 +1.5 38,425 - Ac3H11_1345 0.87 -0.6 38,425 - Ac3H11_1345 0.87 +0.4 38,425 - Ac3H11_1345 0.87 +0.2 38,508 + Ac3H11_1345 0.89 +0.5 38,509 - Ac3H11_1345 0.89 -0.4 38,613 + -1.7 38,613 + +0.6 38,614 - +1.5 38,614 - -0.3 38,614 - -1.4 38,614 - -0.5 38,614 - +0.8 38,977 + +0.7 39,727 + Ac3H11_1346 0.86 +0.1 39,800 - +1.3 39,881 + -0.4 39,882 - +0.3 39,882 - +2.2 39,882 - -0.6 39,939 + -1.0 39,940 - -2.0 39,945 + +0.2 39,945 + -1.1 39,946 - -0.7 40,072 + Ac3H11_1347 0.21 -0.1 40,072 + Ac3H11_1347 0.21 +1.2 40,072 + Ac3H11_1347 0.21 -0.9 40,073 - Ac3H11_1347 0.21 +1.0 40,073 - Ac3H11_1347 0.21 +2.5 40,073 - Ac3H11_1347 0.21 -0.7 40,087 + Ac3H11_1347 0.26 -0.2 40,087 + Ac3H11_1347 0.26 +0.9 40,087 + Ac3H11_1347 0.26 -2.3 40,087 + Ac3H11_1347 0.26 +0.1 40,087 + Ac3H11_1347 0.26 -0.1 40,087 + Ac3H11_1347 0.26 +5.4 40,088 - Ac3H11_1347 0.26 +0.3
Or see this region's nucleotide sequence