Strain Fitness in Acidovorax sp. GW101-3H11 around Ac3H11_101

Experiment: inner cut, R2A soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAc3H11_99 and Ac3H11_100 are separated by 60 nucleotidesAc3H11_100 and Ac3H11_101 are separated by 68 nucleotidesAc3H11_101 and Ac3H11_102 are separated by 34 nucleotidesAc3H11_102 and Ac3H11_103 are separated by 1 nucleotides Ac3H11_99 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 102,336 to 103,883 _99 Ac3H11_100 - Enoyl-CoA hydratase (EC 4.2.1.17), at 103,944 to 104,756 _100 Ac3H11_101 - Benzoate transport, extracellular ligand-binding receptor, at 104,825 to 105,994 _101 Ac3H11_102 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 106,029 to 106,910 _102 Ac3H11_103 - Benzoate transport, inner-membrane translocator precursor, at 106,912 to 107,961 _103 Position (kb) 104 105 106Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 104.015 kb on - strandat 104.015 kb on - strandat 104.361 kb on + strand, within Ac3H11_100at 104.362 kb on - strand, within Ac3H11_100at 104.436 kb on - strand, within Ac3H11_100at 104.506 kb on + strand, within Ac3H11_100at 104.506 kb on + strand, within Ac3H11_100at 104.597 kb on + strand, within Ac3H11_100at 104.597 kb on + strand, within Ac3H11_100at 104.670 kb on + strand, within Ac3H11_100at 104.684 kb on + strandat 104.684 kb on + strandat 104.684 kb on + strandat 104.685 kb on - strandat 104.823 kb on - strandat 104.823 kb on - strandat 104.823 kb on - strandat 104.867 kb on - strandat 104.936 kb on + strandat 104.936 kb on + strandat 104.936 kb on + strandat 104.936 kb on + strandat 104.936 kb on + strandat 104.937 kb on - strandat 104.937 kb on - strandat 104.937 kb on - strandat 104.937 kb on - strandat 105.036 kb on - strand, within Ac3H11_101at 105.266 kb on - strand, within Ac3H11_101at 105.266 kb on - strand, within Ac3H11_101at 105.266 kb on - strand, within Ac3H11_101at 105.326 kb on + strand, within Ac3H11_101at 105.327 kb on - strand, within Ac3H11_101at 105.327 kb on - strand, within Ac3H11_101at 105.443 kb on + strand, within Ac3H11_101at 105.443 kb on + strand, within Ac3H11_101at 105.443 kb on + strand, within Ac3H11_101at 105.444 kb on - strand, within Ac3H11_101at 105.491 kb on + strand, within Ac3H11_101at 105.492 kb on - strand, within Ac3H11_101at 105.492 kb on - strand, within Ac3H11_101at 105.653 kb on + strand, within Ac3H11_101at 105.749 kb on + strand, within Ac3H11_101at 105.749 kb on + strand, within Ac3H11_101at 105.750 kb on - strand, within Ac3H11_101at 105.750 kb on - strand, within Ac3H11_101at 105.767 kb on + strand, within Ac3H11_101at 106.029 kb on - strandat 106.048 kb on + strandat 106.049 kb on - strandat 106.167 kb on + strand, within Ac3H11_102at 106.167 kb on + strand, within Ac3H11_102at 106.174 kb on + strand, within Ac3H11_102at 106.182 kb on + strand, within Ac3H11_102at 106.182 kb on + strand, within Ac3H11_102at 106.182 kb on + strand, within Ac3H11_102at 106.182 kb on + strand, within Ac3H11_102at 106.183 kb on - strand, within Ac3H11_102at 106.183 kb on - strand, within Ac3H11_102at 106.458 kb on + strand, within Ac3H11_102at 106.557 kb on + strand, within Ac3H11_102at 106.557 kb on + strand, within Ac3H11_102at 106.606 kb on - strand, within Ac3H11_102at 106.671 kb on + strand, within Ac3H11_102at 106.672 kb on - strand, within Ac3H11_102at 106.809 kb on + strand, within Ac3H11_102at 106.810 kb on - strand, within Ac3H11_102at 106.810 kb on - strand, within Ac3H11_102

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay
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104,015 - -0.9
104,015 - -0.9
104,361 + Ac3H11_100 0.51 -2.1
104,362 - Ac3H11_100 0.51 -3.3
104,436 - Ac3H11_100 0.61 -1.6
104,506 + Ac3H11_100 0.69 -0.5
104,506 + Ac3H11_100 0.69 -0.3
104,597 + Ac3H11_100 0.80 -0.9
104,597 + Ac3H11_100 0.80 +0.5
104,670 + Ac3H11_100 0.89 +3.0
104,684 + -1.2
104,684 + -1.9
104,684 + -0.6
104,685 - -0.3
104,823 - +1.6
104,823 - -1.1
104,823 - -0.7
104,867 - -1.7
104,936 + +1.8
104,936 + -0.6
104,936 + -0.5
104,936 + -0.5
104,936 + +0.7
104,937 - -1.7
104,937 - -0.1
104,937 - -1.2
104,937 - +0.4
105,036 - Ac3H11_101 0.18 -0.0
105,266 - Ac3H11_101 0.38 -2.0
105,266 - Ac3H11_101 0.38 +0.5
105,266 - Ac3H11_101 0.38 +0.3
105,326 + Ac3H11_101 0.43 -0.5
105,327 - Ac3H11_101 0.43 -0.5
105,327 - Ac3H11_101 0.43 -1.3
105,443 + Ac3H11_101 0.53 +0.4
105,443 + Ac3H11_101 0.53 -0.2
105,443 + Ac3H11_101 0.53 +1.0
105,444 - Ac3H11_101 0.53 -0.5
105,491 + Ac3H11_101 0.57 +0.1
105,492 - Ac3H11_101 0.57 -0.9
105,492 - Ac3H11_101 0.57 +0.9
105,653 + Ac3H11_101 0.71 +1.4
105,749 + Ac3H11_101 0.79 -1.8
105,749 + Ac3H11_101 0.79 +0.7
105,750 - Ac3H11_101 0.79 +2.0
105,750 - Ac3H11_101 0.79 -0.6
105,767 + Ac3H11_101 0.81 +0.2
106,029 - +1.1
106,048 + -2.6
106,049 - -2.0
106,167 + Ac3H11_102 0.16 -0.8
106,167 + Ac3H11_102 0.16 -0.4
106,174 + Ac3H11_102 0.16 +1.0
106,182 + Ac3H11_102 0.17 +0.7
106,182 + Ac3H11_102 0.17 -0.6
106,182 + Ac3H11_102 0.17 -0.8
106,182 + Ac3H11_102 0.17 -0.6
106,183 - Ac3H11_102 0.17 +1.0
106,183 - Ac3H11_102 0.17 -2.9
106,458 + Ac3H11_102 0.49 -1.7
106,557 + Ac3H11_102 0.60 +0.8
106,557 + Ac3H11_102 0.60 -1.2
106,606 - Ac3H11_102 0.65 +1.7
106,671 + Ac3H11_102 0.73 +0.8
106,672 - Ac3H11_102 0.73 -0.3
106,809 + Ac3H11_102 0.88 +1.9
106,810 - Ac3H11_102 0.89 +1.2
106,810 - Ac3H11_102 0.89 -0.4

Or see this region's nucleotide sequence