Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23495 and AO353_23500 are separated by 91 nucleotides AO353_23500 and AO353_23505 are separated by 9 nucleotides AO353_23505 and AO353_23510 are separated by 107 nucleotides AO353_23510 and AO353_23515 are separated by 127 nucleotides
AO353_23495: AO353_23495 - SAM-dependent methyltransferase, at 5,147,831 to 5,148,589
_23495
AO353_23500: AO353_23500 - ribonuclease H, at 5,148,681 to 5,149,133
_23500
AO353_23505: AO353_23505 - hypothetical protein, at 5,149,143 to 5,149,457
_23505
AO353_23510: AO353_23510 - transcriptional regulator, at 5,149,565 to 5,150,455
_23510
AO353_23515: AO353_23515 - muconate cycloisomerase, at 5,150,583 to 5,151,731
_23515
Position (kb)
5149
5150
5151 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5148.590 kb on + strand at 5148.591 kb on - strand at 5148.599 kb on + strand at 5148.599 kb on + strand at 5148.600 kb on - strand at 5148.600 kb on - strand at 5148.694 kb on + strand at 5148.694 kb on + strand at 5148.694 kb on + strand at 5148.695 kb on - strand at 5148.695 kb on - strand at 5148.695 kb on - strand at 5148.874 kb on + strand, within AO353_23500 at 5148.897 kb on + strand, within AO353_23500 at 5149.119 kb on + strand at 5149.119 kb on + strand at 5149.184 kb on - strand, within AO353_23505 at 5149.328 kb on + strand, within AO353_23505 at 5149.328 kb on + strand, within AO353_23505 at 5149.434 kb on + strand at 5149.434 kb on + strand at 5149.434 kb on + strand at 5149.435 kb on - strand at 5149.435 kb on - strand at 5149.435 kb on - strand at 5149.435 kb on - strand at 5149.436 kb on + strand at 5149.436 kb on + strand at 5149.436 kb on + strand at 5149.437 kb on - strand at 5149.482 kb on + strand at 5149.524 kb on + strand at 5149.524 kb on + strand at 5149.525 kb on - strand at 5149.525 kb on - strand at 5149.525 kb on - strand at 5149.546 kb on + strand at 5149.821 kb on + strand, within AO353_23510 at 5149.854 kb on + strand, within AO353_23510 at 5149.854 kb on + strand, within AO353_23510 at 5149.854 kb on + strand, within AO353_23510 at 5149.855 kb on - strand, within AO353_23510 at 5149.855 kb on - strand, within AO353_23510 at 5149.879 kb on - strand, within AO353_23510 at 5149.979 kb on + strand, within AO353_23510 at 5150.008 kb on + strand, within AO353_23510 at 5150.035 kb on + strand, within AO353_23510 at 5150.036 kb on - strand, within AO353_23510 at 5150.152 kb on - strand, within AO353_23510 at 5150.434 kb on - strand at 5150.434 kb on - strand at 5150.513 kb on + strand at 5150.514 kb on - strand at 5150.514 kb on - strand at 5150.699 kb on - strand, within AO353_23515 at 5150.763 kb on - strand, within AO353_23515 at 5150.837 kb on + strand, within AO353_23515 at 5150.838 kb on - strand, within AO353_23515 at 5150.838 kb on - strand, within AO353_23515 at 5150.874 kb on - strand, within AO353_23515 at 5150.885 kb on + strand, within AO353_23515 at 5150.886 kb on - strand, within AO353_23515 at 5150.886 kb on - strand, within AO353_23515 at 5150.894 kb on + strand, within AO353_23515 at 5150.895 kb on - strand, within AO353_23515 at 5150.977 kb on + strand, within AO353_23515 at 5150.978 kb on - strand, within AO353_23515
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 5,148,590 + +0.4 5,148,591 - -0.7 5,148,599 + -0.5 5,148,599 + +1.1 5,148,600 - -0.7 5,148,600 - -0.8 5,148,694 + -0.6 5,148,694 + -1.1 5,148,694 + +0.5 5,148,695 - -1.1 5,148,695 - -1.6 5,148,695 - -0.1 5,148,874 + AO353_23500 0.43 -0.8 5,148,897 + AO353_23500 0.48 -1.6 5,149,119 + +2.2 5,149,119 + -0.1 5,149,184 - AO353_23505 0.13 -1.6 5,149,328 + AO353_23505 0.59 -1.6 5,149,328 + AO353_23505 0.59 -1.4 5,149,434 + -0.1 5,149,434 + +1.2 5,149,434 + +0.1 5,149,435 - +1.0 5,149,435 - -0.7 5,149,435 - +0.2 5,149,435 - +0.4 5,149,436 + -0.4 5,149,436 + +0.3 5,149,436 + +1.6 5,149,437 - -0.8 5,149,482 + +0.7 5,149,524 + +1.0 5,149,524 + -0.8 5,149,525 - +0.4 5,149,525 - +1.2 5,149,525 - +0.4 5,149,546 + -0.2 5,149,821 + AO353_23510 0.29 -0.9 5,149,854 + AO353_23510 0.32 +0.6 5,149,854 + AO353_23510 0.32 -2.5 5,149,854 + AO353_23510 0.32 -0.4 5,149,855 - AO353_23510 0.33 -0.4 5,149,855 - AO353_23510 0.33 -1.4 5,149,879 - AO353_23510 0.35 -0.4 5,149,979 + AO353_23510 0.46 -0.5 5,150,008 + AO353_23510 0.50 -2.8 5,150,035 + AO353_23510 0.53 -0.2 5,150,036 - AO353_23510 0.53 +0.8 5,150,152 - AO353_23510 0.66 -2.5 5,150,434 - -0.2 5,150,434 - -2.4 5,150,513 + +0.3 5,150,514 - +1.0 5,150,514 - -0.8 5,150,699 - AO353_23515 0.10 -0.4 5,150,763 - AO353_23515 0.16 +0.7 5,150,837 + AO353_23515 0.22 +0.0 5,150,838 - AO353_23515 0.22 +0.1 5,150,838 - AO353_23515 0.22 +0.7 5,150,874 - AO353_23515 0.25 +0.9 5,150,885 + AO353_23515 0.26 -0.4 5,150,886 - AO353_23515 0.26 -1.0 5,150,886 - AO353_23515 0.26 -0.0 5,150,894 + AO353_23515 0.27 +0.7 5,150,895 - AO353_23515 0.27 -0.0 5,150,977 + AO353_23515 0.34 -0.1 5,150,978 - AO353_23515 0.34 -0.5
Or see this region's nucleotide sequence