Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_01995 and AO353_02000 are separated by 16 nucleotides AO353_02000 and AO353_02005 overlap by 11 nucleotides AO353_02005 and AO353_02010 are separated by 32 nucleotides
AO353_01995: AO353_01995 - hypothetical protein, at 422,628 to 426,335
_01995
AO353_02000: AO353_02000 - polysaccharide/polyol phosphate ABC transporter ATP-binding protein, at 426,352 to 427,641
_02000
AO353_02005: AO353_02005 - hypothetical protein, at 427,631 to 428,440
_02005
AO353_02010: AO353_02010 - glycosyl transferase family 1, at 428,473 to 429,720
_02010
Position (kb) 426
427
428 Strain fitness (log2 ratio) -2
-1
0
1
2 at 425.404 kb on + strand, within AO353_01995 at 425.404 kb on + strand, within AO353_01995 at 425.426 kb on + strand, within AO353_01995 at 425.521 kb on + strand, within AO353_01995 at 425.789 kb on + strand at 426.354 kb on - strand at 426.354 kb on - strand at 426.598 kb on - strand, within AO353_02000 at 426.749 kb on - strand, within AO353_02000 at 426.749 kb on - strand, within AO353_02000 at 426.751 kb on - strand, within AO353_02000 at 426.868 kb on - strand, within AO353_02000 at 426.911 kb on - strand, within AO353_02000 at 426.957 kb on - strand, within AO353_02000 at 426.961 kb on - strand, within AO353_02000 at 426.988 kb on - strand, within AO353_02000 at 427.159 kb on - strand, within AO353_02000 at 427.503 kb on + strand, within AO353_02000 at 427.504 kb on - strand, within AO353_02000 at 427.584 kb on - strand at 427.584 kb on - strand at 427.587 kb on + strand at 427.988 kb on + strand, within AO353_02005 at 427.989 kb on - strand, within AO353_02005 at 428.132 kb on - strand, within AO353_02005 at 428.387 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 425,404 + AO353_01995 0.75 +0.8 425,404 + AO353_01995 0.75 -0.5 425,426 + AO353_01995 0.75 +2.1 425,521 + AO353_01995 0.78 +0.5 425,789 + -0.1 426,354 - +1.0 426,354 - +0.2 426,598 - AO353_02000 0.19 +0.7 426,749 - AO353_02000 0.31 -2.2 426,749 - AO353_02000 0.31 -0.8 426,751 - AO353_02000 0.31 -1.5 426,868 - AO353_02000 0.40 +0.2 426,911 - AO353_02000 0.43 +0.7 426,957 - AO353_02000 0.47 -1.1 426,961 - AO353_02000 0.47 -1.1 426,988 - AO353_02000 0.49 -0.1 427,159 - AO353_02000 0.63 -0.4 427,503 + AO353_02000 0.89 -2.0 427,504 - AO353_02000 0.89 +0.4 427,584 - -2.3 427,584 - -0.3 427,587 + -0.5 427,988 + AO353_02005 0.44 +0.2 427,989 - AO353_02005 0.44 +0.3 428,132 - AO353_02005 0.62 -2.3 428,387 - +0.7
Or see this region's nucleotide sequence