Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3544 and rbn overlap by 4 nucleotides rbn and Shew_3546 are separated by 155 nucleotides Shew_3546 and Shew_3547 are separated by 24 nucleotides
Shew_3544: Shew_3544 - alpha/beta hydrolase fold (RefSeq), at 4,231,681 to 4,232,631
_3544
Shew_3545: rbn - ribonuclease BN (RefSeq), at 4,232,628 to 4,233,485
rbn
Shew_3546: Shew_3546 - hypothetical protein (RefSeq), at 4,233,641 to 4,233,862
_3546
Shew_3547: Shew_3547 - hypothetical protein (RefSeq), at 4,233,887 to 4,234,537
_3547
Position (kb)
4232
4233
4234 Strain fitness (log2 ratio)
-2
-1
0
1 at 4232.002 kb on - strand, within Shew_3544 at 4232.031 kb on + strand, within Shew_3544 at 4232.170 kb on + strand, within Shew_3544 at 4232.170 kb on + strand, within Shew_3544 at 4232.189 kb on + strand, within Shew_3544 at 4232.287 kb on + strand, within Shew_3544 at 4232.295 kb on - strand, within Shew_3544 at 4232.408 kb on + strand, within Shew_3544 at 4232.521 kb on + strand, within Shew_3544 at 4232.932 kb on - strand, within rbn at 4233.053 kb on + strand, within rbn at 4233.099 kb on + strand, within rbn at 4233.235 kb on + strand, within rbn at 4233.235 kb on + strand, within rbn at 4233.243 kb on - strand, within rbn at 4233.271 kb on + strand, within rbn at 4233.371 kb on + strand, within rbn at 4233.379 kb on - strand, within rbn at 4233.416 kb on - strand at 4233.701 kb on + strand, within Shew_3546 at 4233.740 kb on + strand, within Shew_3546 at 4233.776 kb on - strand, within Shew_3546 at 4233.808 kb on - strand, within Shew_3546 at 4233.847 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 4,232,002 - Shew_3544 0.34 +0.2 4,232,031 + Shew_3544 0.37 -1.2 4,232,170 + Shew_3544 0.51 +0.6 4,232,170 + Shew_3544 0.51 -2.5 4,232,189 + Shew_3544 0.53 +1.1 4,232,287 + Shew_3544 0.64 -0.1 4,232,295 - Shew_3544 0.65 -0.1 4,232,408 + Shew_3544 0.76 +0.0 4,232,521 + Shew_3544 0.88 -0.9 4,232,932 - rbn Shew_3545 0.35 -1.2 4,233,053 + rbn Shew_3545 0.50 -0.7 4,233,099 + rbn Shew_3545 0.55 -0.2 4,233,235 + rbn Shew_3545 0.71 -1.1 4,233,235 + rbn Shew_3545 0.71 -1.4 4,233,243 - rbn Shew_3545 0.72 -0.8 4,233,271 + rbn Shew_3545 0.75 -0.6 4,233,371 + rbn Shew_3545 0.87 -1.8 4,233,379 - rbn Shew_3545 0.88 -1.9 4,233,416 - -0.9 4,233,701 + Shew_3546 0.27 -0.3 4,233,740 + Shew_3546 0.45 +1.3 4,233,776 - Shew_3546 0.61 +0.0 4,233,808 - Shew_3546 0.75 +0.2 4,233,847 + +0.2
Or see this region's nucleotide sequence