Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3221 and Shew_3222 are separated by 72 nucleotides Shew_3222 and Shew_3223 are separated by 175 nucleotides Shew_3223 and Shew_3224 are separated by 3 nucleotides
Shew_3221: Shew_3221 - hypothetical protein (RefSeq), at 3,844,542 to 3,845,702
_3221
Shew_3222: Shew_3222 - protein of unknown function DUF335, SprT (RefSeq), at 3,845,775 to 3,846,386
_3222
Shew_3223: Shew_3223 - deoxyribonuclease I (RefSeq), at 3,846,562 to 3,847,317
_3223
Shew_3224: Shew_3224 - hypothetical protein (RefSeq), at 3,847,321 to 3,848,052
_3224
Position (kb)
3845
3846
3847 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 3844.854 kb on - strand, within Shew_3221 at 3844.894 kb on + strand, within Shew_3221 at 3845.024 kb on - strand, within Shew_3221 at 3845.087 kb on + strand, within Shew_3221 at 3845.161 kb on + strand, within Shew_3221 at 3845.165 kb on + strand, within Shew_3221 at 3845.306 kb on + strand, within Shew_3221 at 3845.394 kb on + strand, within Shew_3221 at 3845.441 kb on + strand, within Shew_3221 at 3845.449 kb on - strand, within Shew_3221 at 3845.498 kb on - strand, within Shew_3221 at 3846.041 kb on + strand, within Shew_3222 at 3846.089 kb on + strand, within Shew_3222 at 3846.202 kb on + strand, within Shew_3222 at 3846.237 kb on + strand, within Shew_3222 at 3846.670 kb on - strand, within Shew_3223 at 3847.082 kb on - strand, within Shew_3223 at 3847.254 kb on + strand at 3847.275 kb on - strand at 3847.352 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 3,844,854 - Shew_3221 0.27 +5.7 3,844,894 + Shew_3221 0.30 +5.9 3,845,024 - Shew_3221 0.42 +5.2 3,845,087 + Shew_3221 0.47 +0.2 3,845,161 + Shew_3221 0.53 +1.2 3,845,165 + Shew_3221 0.54 +0.1 3,845,306 + Shew_3221 0.66 -0.2 3,845,394 + Shew_3221 0.73 -0.2 3,845,441 + Shew_3221 0.77 +0.2 3,845,449 - Shew_3221 0.78 +4.1 3,845,498 - Shew_3221 0.82 +4.2 3,846,041 + Shew_3222 0.43 +0.7 3,846,089 + Shew_3222 0.51 +1.0 3,846,202 + Shew_3222 0.70 +0.5 3,846,237 + Shew_3222 0.75 +1.4 3,846,670 - Shew_3223 0.14 +0.6 3,847,082 - Shew_3223 0.69 -0.0 3,847,254 + -0.2 3,847,275 - -1.1 3,847,352 + +0.1
Or see this region's nucleotide sequence