Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3220 and Shew_3221 are separated by 409 nucleotides Shew_3221 and Shew_3222 are separated by 72 nucleotides Shew_3222 and Shew_3223 are separated by 175 nucleotides
Shew_3220: Shew_3220 - Beta-lactamase (RefSeq), at 3,843,326 to 3,844,132
_3220
Shew_3221: Shew_3221 - hypothetical protein (RefSeq), at 3,844,542 to 3,845,702
_3221
Shew_3222: Shew_3222 - protein of unknown function DUF335, SprT (RefSeq), at 3,845,775 to 3,846,386
_3222
Shew_3223: Shew_3223 - deoxyribonuclease I (RefSeq), at 3,846,562 to 3,847,317
_3223
Position (kb)
3844
3845
3846 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 3843.656 kb on - strand, within Shew_3220 at 3843.819 kb on - strand, within Shew_3220 at 3843.866 kb on - strand, within Shew_3220 at 3844.068 kb on - strand at 3844.092 kb on + strand at 3844.147 kb on + strand at 3844.169 kb on + strand at 3844.177 kb on - strand at 3844.189 kb on - strand at 3844.241 kb on + strand at 3844.249 kb on - strand at 3844.414 kb on + strand at 3844.501 kb on - strand at 3844.501 kb on - strand at 3844.501 kb on - strand at 3844.505 kb on + strand at 3844.505 kb on + strand at 3844.610 kb on - strand at 3844.628 kb on + strand at 3844.854 kb on - strand, within Shew_3221 at 3844.894 kb on + strand, within Shew_3221 at 3845.024 kb on - strand, within Shew_3221 at 3845.087 kb on + strand, within Shew_3221 at 3845.161 kb on + strand, within Shew_3221 at 3845.165 kb on + strand, within Shew_3221 at 3845.306 kb on + strand, within Shew_3221 at 3845.394 kb on + strand, within Shew_3221 at 3845.441 kb on + strand, within Shew_3221 at 3845.449 kb on - strand, within Shew_3221 at 3845.498 kb on - strand, within Shew_3221 at 3846.041 kb on + strand, within Shew_3222 at 3846.089 kb on + strand, within Shew_3222 at 3846.202 kb on + strand, within Shew_3222 at 3846.237 kb on + strand, within Shew_3222 at 3846.670 kb on - strand, within Shew_3223
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 3,843,656 - Shew_3220 0.41 -2.8 3,843,819 - Shew_3220 0.61 -3.8 3,843,866 - Shew_3220 0.67 -3.5 3,844,068 - -2.7 3,844,092 + -4.4 3,844,147 + -4.0 3,844,169 + -5.0 3,844,177 - -4.2 3,844,189 - -0.9 3,844,241 + -2.4 3,844,249 - -3.0 3,844,414 + +0.2 3,844,501 - +5.9 3,844,501 - +5.8 3,844,501 - +5.1 3,844,505 + +0.3 3,844,505 + +0.1 3,844,610 - +5.5 3,844,628 + +0.0 3,844,854 - Shew_3221 0.27 +5.7 3,844,894 + Shew_3221 0.30 +5.9 3,845,024 - Shew_3221 0.42 +5.2 3,845,087 + Shew_3221 0.47 +0.2 3,845,161 + Shew_3221 0.53 +1.2 3,845,165 + Shew_3221 0.54 +0.1 3,845,306 + Shew_3221 0.66 -0.2 3,845,394 + Shew_3221 0.73 -0.2 3,845,441 + Shew_3221 0.77 +0.2 3,845,449 - Shew_3221 0.78 +4.1 3,845,498 - Shew_3221 0.82 +4.2 3,846,041 + Shew_3222 0.43 +0.7 3,846,089 + Shew_3222 0.51 +1.0 3,846,202 + Shew_3222 0.70 +0.5 3,846,237 + Shew_3222 0.75 +1.4 3,846,670 - Shew_3223 0.14 +0.6
Or see this region's nucleotide sequence