Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt napG and napH overlap by 4 nucleotides napH and Shew_3209 overlap by 4 nucleotides Shew_3209 and Shew_3210 are separated by 529 nucleotides Shew_3210 and Shew_3211 are separated by 52 nucleotides
Shew_3207: napG - quinol dehydrogenase periplasmic component (RefSeq), at 3,826,150 to 3,826,881
napG
Shew_3208: napH - quinol dehydrogenase membrane component (RefSeq), at 3,826,878 to 3,827,777
napH
Shew_3209: Shew_3209 - nitrate reductase cytochrome c-type subunit (NapB) (RefSeq), at 3,827,774 to 3,828,196
_3209
Shew_3210: Shew_3210 - hypothetical protein (RefSeq), at 3,828,726 to 3,829,013
_3210
Shew_3211: Shew_3211 - glutathione S-transferase domain-containing protein (RefSeq), at 3,829,066 to 3,829,662
_3211
Position (kb)
3827
3828
3829 Strain fitness (log2 ratio)
-2
-1
0
1 at 3826.776 kb on + strand, within napG at 3826.847 kb on + strand at 3827.151 kb on + strand, within napH at 3827.398 kb on - strand, within napH at 3827.503 kb on - strand, within napH at 3827.505 kb on + strand, within napH at 3827.538 kb on - strand, within napH at 3827.615 kb on + strand, within napH at 3827.911 kb on - strand, within Shew_3209 at 3827.969 kb on + strand, within Shew_3209 at 3828.642 kb on + strand at 3828.833 kb on + strand, within Shew_3210 at 3828.911 kb on - strand, within Shew_3210 at 3829.017 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 3,826,776 + napG Shew_3207 0.86 -0.0 3,826,847 + -0.2 3,827,151 + napH Shew_3208 0.30 -1.3 3,827,398 - napH Shew_3208 0.58 +0.2 3,827,503 - napH Shew_3208 0.69 -0.1 3,827,505 + napH Shew_3208 0.70 -0.5 3,827,538 - napH Shew_3208 0.73 -2.4 3,827,615 + napH Shew_3208 0.82 -0.2 3,827,911 - Shew_3209 0.32 +0.7 3,827,969 + Shew_3209 0.46 +0.5 3,828,642 + -0.3 3,828,833 + Shew_3210 0.37 +0.3 3,828,911 - Shew_3210 0.64 +0.6 3,829,017 + -0.2
Or see this region's nucleotide sequence