Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2960 and Shew_2961 are separated by 81 nucleotides Shew_2961 and Shew_2962 are separated by 290 nucleotides Shew_2962 and Shew_2963 are separated by 210 nucleotides Shew_2963 and Shew_2964 are separated by 55 nucleotides
Shew_2960: Shew_2960 - peptidase S8 and S53, subtilisin, kexin, sedolisin (RefSeq), at 3,508,399 to 3,513,378
_2960
Shew_2961: Shew_2961 - hypothetical protein (RefSeq), at 3,513,460 to 3,513,693
_2961
Shew_2962: Shew_2962 - malate dehydrogenase (RefSeq), at 3,513,984 to 3,515,672
_2962
Shew_2963: Shew_2963 - hypothetical protein (RefSeq), at 3,515,883 to 3,516,347
_2963
Shew_2964: Shew_2964 - beta-lactamase (RefSeq), at 3,516,403 to 3,517,545
_2964
Position (kb)
3513
3514
3515
3516 Strain fitness (log2 ratio)
-2
-1
0
1 at 3513.232 kb on - strand at 3513.259 kb on - strand at 3513.289 kb on - strand at 3513.501 kb on + strand, within Shew_2961 at 3513.509 kb on - strand, within Shew_2961 at 3513.509 kb on - strand, within Shew_2961 at 3513.509 kb on - strand, within Shew_2961 at 3513.509 kb on - strand, within Shew_2961 at 3513.706 kb on + strand at 3514.021 kb on + strand at 3514.302 kb on - strand, within Shew_2962 at 3514.877 kb on + strand, within Shew_2962 at 3515.101 kb on - strand, within Shew_2962 at 3515.101 kb on - strand, within Shew_2962 at 3515.120 kb on - strand, within Shew_2962 at 3515.431 kb on - strand, within Shew_2962 at 3515.503 kb on - strand, within Shew_2962 at 3515.817 kb on + strand at 3516.053 kb on + strand, within Shew_2963 at 3516.181 kb on - strand, within Shew_2963 at 3516.580 kb on - strand, within Shew_2964 at 3516.604 kb on + strand, within Shew_2964 at 3516.635 kb on - strand, within Shew_2964
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 3,513,232 - +0.4 3,513,259 - -1.1 3,513,289 - +0.4 3,513,501 + Shew_2961 0.18 +0.5 3,513,509 - Shew_2961 0.21 +0.9 3,513,509 - Shew_2961 0.21 -0.3 3,513,509 - Shew_2961 0.21 +0.2 3,513,509 - Shew_2961 0.21 +0.6 3,513,706 + +0.2 3,514,021 + -0.3 3,514,302 - Shew_2962 0.19 -0.3 3,514,877 + Shew_2962 0.53 -1.8 3,515,101 - Shew_2962 0.66 -1.0 3,515,101 - Shew_2962 0.66 -0.8 3,515,120 - Shew_2962 0.67 +0.4 3,515,431 - Shew_2962 0.86 +0.4 3,515,503 - Shew_2962 0.90 -0.3 3,515,817 + +0.2 3,516,053 + Shew_2963 0.37 +0.1 3,516,181 - Shew_2963 0.64 +0.4 3,516,580 - Shew_2964 0.15 -0.3 3,516,604 + Shew_2964 0.18 +0.2 3,516,635 - Shew_2964 0.20 -0.5
Or see this region's nucleotide sequence