Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2087 and Shew_2088 are separated by 15 nucleotides Shew_2088 and Shew_2089 overlap by 24 nucleotides Shew_2089 and Shew_2090 are separated by 3 nucleotides Shew_2090 and thiH are separated by 102 nucleotides
Shew_2087: Shew_2087 - hypothetical protein (RefSeq), at 2,414,398 to 2,415,549
_2087
Shew_2088: Shew_2088 - LysR family transcriptional regulator (RefSeq), at 2,415,565 to 2,416,476
_2088
Shew_2089: Shew_2089 - SrpA-related protein (RefSeq), at 2,416,453 to 2,417,850
_2089
Shew_2090: Shew_2090 - methyl-accepting chemotaxis protein (RefSeq), at 2,417,854 to 2,418,123
_2090
Shew_2091: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,418,226 to 2,419,344
thiH
Position (kb) 2416
2417
2418 Strain fitness (log2 ratio) -1
0
1 at 2415.492 kb on + strand at 2416.197 kb on - strand, within Shew_2088 at 2416.450 kb on + strand at 2416.888 kb on - strand, within Shew_2089 at 2417.080 kb on - strand, within Shew_2089 at 2417.296 kb on - strand, within Shew_2089 at 2418.052 kb on + strand, within Shew_2090
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 2,415,492 + +0.4 2,416,197 - Shew_2088 0.69 -0.5 2,416,450 + +1.4 2,416,888 - Shew_2089 0.31 +0.8 2,417,080 - Shew_2089 0.45 +0.4 2,417,296 - Shew_2089 0.60 +0.7 2,418,052 + Shew_2090 0.73 -0.4
Or see this region's nucleotide sequence