Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1982 and Shew_1983 are separated by 322 nucleotides Shew_1983 and Shew_1984 are separated by 192 nucleotides Shew_1984 and Shew_1985 are separated by 264 nucleotides
Shew_1982: Shew_1982 - phage integrase family protein (RefSeq), at 2,293,640 to 2,294,911
_1982
Shew_1983: Shew_1983 - response regulator receiver modulated metal dependent phosphohydrolase (RefSeq), at 2,295,234 to 2,296,805
_1983
Shew_1984: Shew_1984 - hypothetical protein (RefSeq), at 2,296,998 to 2,297,495
_1984
Shew_1985: Shew_1985 - cytochrome c oxidase, cbb3-type, subunit I (RefSeq), at 2,297,760 to 2,299,187
_1985
Position (kb)
2295
2296
2297 Strain fitness (log2 ratio)
-1
0
1 at 2294.272 kb on - strand, within Shew_1982 at 2294.588 kb on - strand, within Shew_1982 at 2294.603 kb on - strand, within Shew_1982 at 2294.792 kb on - strand at 2294.812 kb on + strand at 2294.830 kb on - strand at 2295.226 kb on + strand at 2295.273 kb on + strand at 2295.350 kb on - strand at 2295.377 kb on + strand at 2295.471 kb on + strand, within Shew_1983 at 2295.494 kb on - strand, within Shew_1983 at 2295.508 kb on + strand, within Shew_1983 at 2295.685 kb on - strand, within Shew_1983 at 2295.685 kb on - strand, within Shew_1983 at 2295.789 kb on - strand, within Shew_1983 at 2295.789 kb on - strand, within Shew_1983 at 2296.539 kb on - strand, within Shew_1983 at 2296.756 kb on + strand at 2296.764 kb on - strand at 2296.927 kb on + strand at 2296.940 kb on + strand at 2296.967 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 2,294,272 - Shew_1982 0.50 -0.8 2,294,588 - Shew_1982 0.75 -1.1 2,294,603 - Shew_1982 0.76 +0.3 2,294,792 - -0.4 2,294,812 + -0.8 2,294,830 - +0.3 2,295,226 + -0.4 2,295,273 + +0.2 2,295,350 - +0.2 2,295,377 + +0.1 2,295,471 + Shew_1983 0.15 +0.4 2,295,494 - Shew_1983 0.17 +0.4 2,295,508 + Shew_1983 0.17 +0.2 2,295,685 - Shew_1983 0.29 +1.2 2,295,685 - Shew_1983 0.29 +1.1 2,295,789 - Shew_1983 0.35 -0.4 2,295,789 - Shew_1983 0.35 -0.2 2,296,539 - Shew_1983 0.83 +0.9 2,296,756 + +0.5 2,296,764 - +0.7 2,296,927 + +0.0 2,296,940 + -0.1 2,296,967 - +0.4
Or see this region's nucleotide sequence