Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1773 and Shew_1774 are separated by 301 nucleotides Shew_1774 and Shew_1775 are separated by 173 nucleotides Shew_1775 and Shew_1776 overlap by 1 nucleotides
Shew_1773: Shew_1773 - LysR family transcriptional regulator (RefSeq), at 2,027,904 to 2,028,824
_1773
Shew_1774: Shew_1774 - excinuclease ATPase subunit (RefSeq), at 2,029,126 to 2,029,623
_1774
Shew_1775: Shew_1775 - acetolactate synthase 3 regulatory subunit (RefSeq), at 2,029,797 to 2,030,291
_1775
Shew_1776: Shew_1776 - acetolactate synthase 3 catalytic subunit (RefSeq), at 2,030,291 to 2,032,012
_1776
Position (kb)
2029
2030
2031 Strain fitness (log2 ratio)
-2
-1
0
1 at 2028.962 kb on + strand at 2029.290 kb on + strand, within Shew_1774 at 2029.298 kb on - strand, within Shew_1774 at 2029.841 kb on - strand at 2029.922 kb on + strand, within Shew_1775 at 2030.173 kb on - strand, within Shew_1775 at 2030.360 kb on - strand at 2030.654 kb on - strand, within Shew_1776 at 2031.228 kb on - strand, within Shew_1776
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 2,028,962 + -0.5 2,029,290 + Shew_1774 0.33 +0.3 2,029,298 - Shew_1774 0.35 +0.3 2,029,841 - -0.0 2,029,922 + Shew_1775 0.25 +0.1 2,030,173 - Shew_1775 0.76 -2.0 2,030,360 - -0.1 2,030,654 - Shew_1776 0.21 +0.3 2,031,228 - Shew_1776 0.54 +0.7
Or see this region's nucleotide sequence