Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1297 and guaA are separated by 102 nucleotides guaA and Shew_1299 are separated by 146 nucleotides Shew_1299 and Shew_1300 are separated by 233 nucleotides
Shew_1297: Shew_1297 - inositol-5-monophosphate dehydrogenase (RefSeq), at 1,498,544 to 1,500,010
_1297
Shew_1298: guaA - bifunctional GMP synthase/glutamine amidotransferase protein (RefSeq), at 1,500,113 to 1,501,690
guaA
Shew_1299: Shew_1299 - CMP/dCMP deaminase, zinc-binding (RefSeq), at 1,501,837 to 1,502,373
_1299
Shew_1300: Shew_1300 - lytic transglycosylase, catalytic (RefSeq), at 1,502,607 to 1,504,037
_1300
Position (kb)
1500
1501
1502 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1499.995 kb on - strand at 1500.417 kb on - strand, within guaA at 1500.814 kb on + strand, within guaA at 1500.821 kb on + strand, within guaA at 1501.346 kb on + strand, within guaA at 1501.422 kb on + strand, within guaA at 1501.422 kb on + strand, within guaA at 1501.442 kb on - strand, within guaA at 1501.598 kb on + strand at 1501.791 kb on + strand at 1502.544 kb on - strand at 1502.566 kb on + strand at 1502.566 kb on + strand at 1502.676 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 1,499,995 - +3.4 1,500,417 - guaA Shew_1298 0.19 +2.8 1,500,814 + guaA Shew_1298 0.44 +3.6 1,500,821 + guaA Shew_1298 0.45 +2.7 1,501,346 + guaA Shew_1298 0.78 +3.0 1,501,422 + guaA Shew_1298 0.83 +2.9 1,501,422 + guaA Shew_1298 0.83 +4.0 1,501,442 - guaA Shew_1298 0.84 +3.4 1,501,598 + +3.1 1,501,791 + -2.6 1,502,544 - +0.5 1,502,566 + -0.0 1,502,566 + -0.7 1,502,676 - -0.2
Or see this region's nucleotide sequence