Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1265 and Shew_1266 are separated by 4 nucleotides Shew_1266 and Shew_1267 are separated by 538 nucleotides Shew_1267 and ssrA are separated by 201 nucleotides ssrA and smpB are separated by 122 nucleotides
Shew_1265: Shew_1265 - hypothetical protein (RefSeq), at 1,464,090 to 1,464,632
_1265
Shew_1266: Shew_1266 - XRE family transcriptional regulator (RefSeq), at 1,464,637 to 1,465,020
_1266
Shew_1267: Shew_1267 - phage integrase family protein (RefSeq), at 1,465,559 to 1,466,788
_1267
Shew_R0032: ssrA - no description, at 1,466,990 to 1,467,345
ssrA
Shew_1268: smpB - SsrA-binding protein (RefSeq), at 1,467,468 to 1,467,959
smpB
Position (kb) 1465
1466
1467 Strain fitness (log2 ratio) -1
0
1 at 1464.825 kb on + strand, within Shew_1266 at 1465.755 kb on + strand, within Shew_1267 at 1465.763 kb on - strand, within Shew_1267 at 1465.989 kb on - strand, within Shew_1267 at 1466.085 kb on - strand, within Shew_1267 at 1466.143 kb on + strand, within Shew_1267 at 1466.280 kb on - strand, within Shew_1267
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 1,464,825 + Shew_1266 0.49 -0.5 1,465,755 + Shew_1267 0.16 -0.0 1,465,763 - Shew_1267 0.17 +1.3 1,465,989 - Shew_1267 0.35 +0.6 1,466,085 - Shew_1267 0.43 +0.0 1,466,143 + Shew_1267 0.47 +0.2 1,466,280 - Shew_1267 0.59 +0.1
Or see this region's nucleotide sequence