Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1252 and Shew_1253 are separated by 89 nucleotides Shew_1253 and Shew_1254 are separated by 72 nucleotides Shew_1254 and Shew_1255 are separated by 109 nucleotides Shew_1255 and Shew_1256 are separated by 79 nucleotides
Shew_1252: Shew_1252 - transposase (RefSeq), at 1,450,808 to 1,451,473
_1252
Shew_1253: Shew_1253 - retinol acyltransferase domain-containing protein (RefSeq), at 1,451,563 to 1,452,288
_1253
Shew_1254: Shew_1254 - phage integrase family protein (RefSeq), at 1,452,361 to 1,452,921
_1254
Shew_1255: Shew_1255 - hypothetical protein (RefSeq), at 1,453,031 to 1,453,435
_1255
Shew_1256: Shew_1256 - hypothetical protein (RefSeq), at 1,453,515 to 1,453,877
_1256
Position (kb)
1452
1453 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1451.416 kb on + strand at 1451.416 kb on + strand at 1451.476 kb on + strand at 1451.532 kb on + strand at 1451.540 kb on - strand at 1451.559 kb on - strand at 1451.683 kb on - strand, within Shew_1253 at 1451.806 kb on + strand, within Shew_1253 at 1451.811 kb on + strand, within Shew_1253 at 1451.814 kb on - strand, within Shew_1253 at 1451.814 kb on - strand, within Shew_1253 at 1451.814 kb on - strand, within Shew_1253 at 1451.819 kb on - strand, within Shew_1253 at 1451.922 kb on + strand, within Shew_1253 at 1451.931 kb on + strand, within Shew_1253 at 1452.127 kb on - strand, within Shew_1253 at 1452.228 kb on + strand at 1452.228 kb on + strand at 1452.228 kb on + strand at 1452.310 kb on + strand at 1452.320 kb on + strand at 1452.333 kb on + strand at 1452.346 kb on - strand at 1452.536 kb on + strand, within Shew_1254 at 1452.794 kb on + strand, within Shew_1254 at 1452.804 kb on + strand, within Shew_1254 at 1452.804 kb on + strand, within Shew_1254 at 1453.488 kb on - strand at 1453.622 kb on + strand, within Shew_1256 at 1453.630 kb on - strand, within Shew_1256 at 1453.697 kb on - strand, within Shew_1256 at 1453.708 kb on + strand, within Shew_1256 at 1453.708 kb on + strand, within Shew_1256 at 1453.716 kb on - strand, within Shew_1256 at 1453.781 kb on + strand, within Shew_1256 at 1453.795 kb on - strand, within Shew_1256 at 1453.833 kb on - strand, within Shew_1256 at 1453.833 kb on - strand, within Shew_1256
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 1,451,416 + +0.3 1,451,416 + -1.8 1,451,476 + +0.1 1,451,532 + +0.1 1,451,540 - -0.3 1,451,559 - -1.0 1,451,683 - Shew_1253 0.17 +2.0 1,451,806 + Shew_1253 0.33 +0.1 1,451,811 + Shew_1253 0.34 -1.1 1,451,814 - Shew_1253 0.35 +0.3 1,451,814 - Shew_1253 0.35 +0.6 1,451,814 - Shew_1253 0.35 -0.3 1,451,819 - Shew_1253 0.35 +0.0 1,451,922 + Shew_1253 0.49 +1.0 1,451,931 + Shew_1253 0.51 -1.1 1,452,127 - Shew_1253 0.78 +0.3 1,452,228 + +0.9 1,452,228 + +0.5 1,452,228 + -0.3 1,452,310 + +0.1 1,452,320 + -0.4 1,452,333 + +0.2 1,452,346 - -0.3 1,452,536 + Shew_1254 0.31 +0.4 1,452,794 + Shew_1254 0.77 +0.3 1,452,804 + Shew_1254 0.79 -0.0 1,452,804 + Shew_1254 0.79 +3.1 1,453,488 - -0.1 1,453,622 + Shew_1256 0.29 -0.0 1,453,630 - Shew_1256 0.32 +0.5 1,453,697 - Shew_1256 0.50 +0.0 1,453,708 + Shew_1256 0.53 +0.2 1,453,708 + Shew_1256 0.53 +0.3 1,453,716 - Shew_1256 0.55 -0.3 1,453,781 + Shew_1256 0.73 +0.5 1,453,795 - Shew_1256 0.77 +0.3 1,453,833 - Shew_1256 0.88 +0.1 1,453,833 - Shew_1256 0.88 +0.8
Or see this region's nucleotide sequence