Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1172 and Shew_1173 are separated by 43 nucleotides Shew_1173 and Shew_1174 are separated by 193 nucleotides Shew_1174 and Shew_1175 are separated by 171 nucleotides
Shew_1172: Shew_1172 - starch synthase (RefSeq), at 1,354,568 to 1,356,217
_1172
Shew_1173: Shew_1173 - hypothetical protein (RefSeq), at 1,356,261 to 1,356,734
_1173
Shew_1174: Shew_1174 - sulfotransferase (RefSeq), at 1,356,928 to 1,358,523
_1174
Shew_1175: Shew_1175 - SecC motif-containing protein (RefSeq), at 1,358,695 to 1,359,846
_1175
Position (kb)
1356
1357
1358
1359 Strain fitness (log2 ratio)
-1
0
1
2 at 1355.972 kb on + strand, within Shew_1172 at 1355.972 kb on + strand, within Shew_1172 at 1356.187 kb on + strand at 1356.210 kb on - strand at 1356.211 kb on + strand at 1356.220 kb on - strand at 1356.278 kb on - strand at 1356.294 kb on - strand at 1356.600 kb on + strand, within Shew_1173 at 1356.608 kb on - strand, within Shew_1173 at 1356.629 kb on + strand, within Shew_1173 at 1356.756 kb on + strand at 1356.927 kb on - strand at 1357.096 kb on + strand, within Shew_1174 at 1357.936 kb on + strand, within Shew_1174 at 1357.944 kb on - strand, within Shew_1174 at 1358.014 kb on - strand, within Shew_1174 at 1358.014 kb on - strand, within Shew_1174 at 1358.064 kb on + strand, within Shew_1174 at 1358.064 kb on + strand, within Shew_1174 at 1358.293 kb on + strand, within Shew_1174 at 1358.316 kb on + strand, within Shew_1174 at 1358.543 kb on - strand at 1358.766 kb on + strand at 1358.910 kb on - strand, within Shew_1175 at 1359.062 kb on - strand, within Shew_1175 at 1359.162 kb on + strand, within Shew_1175 at 1359.170 kb on - strand, within Shew_1175 at 1359.277 kb on - strand, within Shew_1175 at 1359.474 kb on + strand, within Shew_1175
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 1,355,972 + Shew_1172 0.85 -0.8 1,355,972 + Shew_1172 0.85 +0.1 1,356,187 + -0.2 1,356,210 - -0.0 1,356,211 + -0.5 1,356,220 - +1.8 1,356,278 - -0.2 1,356,294 - +0.4 1,356,600 + Shew_1173 0.72 +0.3 1,356,608 - Shew_1173 0.73 -1.5 1,356,629 + Shew_1173 0.78 -0.3 1,356,756 + -1.2 1,356,927 - +0.2 1,357,096 + Shew_1174 0.11 +0.3 1,357,936 + Shew_1174 0.63 -0.1 1,357,944 - Shew_1174 0.64 +1.2 1,358,014 - Shew_1174 0.68 +1.4 1,358,014 - Shew_1174 0.68 +0.2 1,358,064 + Shew_1174 0.71 +0.4 1,358,064 + Shew_1174 0.71 +0.1 1,358,293 + Shew_1174 0.86 -0.1 1,358,316 + Shew_1174 0.87 +0.3 1,358,543 - -0.9 1,358,766 + +0.0 1,358,910 - Shew_1175 0.19 -0.2 1,359,062 - Shew_1175 0.32 -0.2 1,359,162 + Shew_1175 0.41 +0.2 1,359,170 - Shew_1175 0.41 -0.4 1,359,277 - Shew_1175 0.51 -0.2 1,359,474 + Shew_1175 0.68 -0.1
Or see this region's nucleotide sequence