Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1152 and Shew_1153 are separated by 81 nucleotides Shew_1153 and Shew_1154 are separated by 23 nucleotides Shew_1154 and Shew_1155 are separated by 317 nucleotides
Shew_1152: Shew_1152 - MscS mechanosensitive ion channel (RefSeq), at 1,325,882 to 1,326,706
_1152
Shew_1153: Shew_1153 - no description, at 1,326,788 to 1,326,934
_1153
Shew_1154: Shew_1154 - hypothetical protein (RefSeq), at 1,326,958 to 1,327,611
_1154
Shew_1155: Shew_1155 - threonine aldolase (RefSeq), at 1,327,929 to 1,328,936
_1155
Position (kb)
1326
1327
1328 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1326.098 kb on + strand, within Shew_1152 at 1326.430 kb on + strand, within Shew_1152 at 1326.767 kb on - strand at 1327.098 kb on + strand, within Shew_1154 at 1327.133 kb on - strand, within Shew_1154 at 1327.464 kb on + strand, within Shew_1154 at 1327.861 kb on - strand at 1327.861 kb on - strand at 1328.180 kb on - strand, within Shew_1155 at 1328.186 kb on - strand, within Shew_1155 at 1328.232 kb on - strand, within Shew_1155 at 1328.416 kb on - strand, within Shew_1155 at 1328.507 kb on - strand, within Shew_1155 at 1328.513 kb on + strand, within Shew_1155
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 1,326,098 + Shew_1152 0.26 -0.5 1,326,430 + Shew_1152 0.66 -2.5 1,326,767 - +0.5 1,327,098 + Shew_1154 0.21 -0.6 1,327,133 - Shew_1154 0.27 -0.6 1,327,464 + Shew_1154 0.77 -1.0 1,327,861 - +1.8 1,327,861 - -0.6 1,328,180 - Shew_1155 0.25 +0.1 1,328,186 - Shew_1155 0.25 +0.3 1,328,232 - Shew_1155 0.30 -0.0 1,328,416 - Shew_1155 0.48 +0.2 1,328,507 - Shew_1155 0.57 -0.5 1,328,513 + Shew_1155 0.58 +1.2
Or see this region's nucleotide sequence