Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0828 and Shew_0829 are separated by 100 nucleotides Shew_0829 and Shew_0830 are separated by 13 nucleotides Shew_0830 and Shew_0831 are separated by 40 nucleotides Shew_0831 and Shew_0832 are separated by 358 nucleotides
Shew_0828: Shew_0828 - LysR family transcriptional regulator (RefSeq), at 950,712 to 951,605
_0828
Shew_0829: Shew_0829 - LrgA family protein (RefSeq), at 951,706 to 952,125
_0829
Shew_0830: Shew_0830 - LrgB family protein (RefSeq), at 952,139 to 952,828
_0830
Shew_0831: Shew_0831 - CBS domain-containing protein (RefSeq), at 952,869 to 953,288
_0831
Shew_0832: Shew_0832 - alkaline phosphatase (RefSeq), at 953,647 to 955,419
_0832
Position (kb)
952
953 Strain fitness (log2 ratio)
-1
0
1
2 at 951.502 kb on + strand, within Shew_0828 at 951.549 kb on + strand at 951.987 kb on + strand, within Shew_0829 at 952.010 kb on + strand, within Shew_0829 at 952.010 kb on - strand, within Shew_0829 at 952.018 kb on - strand, within Shew_0829 at 952.196 kb on - strand at 952.196 kb on - strand at 952.435 kb on - strand, within Shew_0830 at 952.501 kb on + strand, within Shew_0830 at 952.763 kb on + strand at 952.903 kb on - strand at 952.903 kb on - strand at 953.524 kb on + strand at 953.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 951,502 + Shew_0828 0.88 -1.4 951,549 + +0.7 951,987 + Shew_0829 0.67 +1.9 952,010 + Shew_0829 0.72 +0.2 952,010 - Shew_0829 0.72 +0.4 952,018 - Shew_0829 0.74 -0.3 952,196 - -0.6 952,196 - +0.6 952,435 - Shew_0830 0.43 -0.4 952,501 + Shew_0830 0.52 +2.1 952,763 + -0.4 952,903 - -0.6 952,903 - +1.1 953,524 + -0.1 953,605 - +1.0
Or see this region's nucleotide sequence