Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0818 and Shew_0819 are separated by 40 nucleotides Shew_0819 and Shew_0820 are separated by 49 nucleotides Shew_0820 and Shew_0821 are separated by 156 nucleotides Shew_0821 and Shew_0822 are separated by 66 nucleotides
Shew_0818: Shew_0818 - peptidase U32 (RefSeq), at 941,285 to 943,198
_0818
Shew_0819: Shew_0819 - hypothetical protein (RefSeq), at 943,239 to 943,613
_0819
Shew_0820: Shew_0820 - hypothetical protein (RefSeq), at 943,663 to 944,850
_0820
Shew_0821: Shew_0821 - mitomycin resistance protein (RefSeq), at 945,007 to 945,294
_0821
Shew_0822: Shew_0822 - lysine exporter protein LysE/YggA (RefSeq), at 945,361 to 945,954
_0822
Position (kb) 943
944
945 Strain fitness (log2 ratio) -1
0
1 at 942.721 kb on - strand, within Shew_0818 at 942.896 kb on + strand, within Shew_0818 at 943.632 kb on - strand at 943.666 kb on + strand at 943.732 kb on - strand at 943.825 kb on - strand, within Shew_0820 at 943.926 kb on + strand, within Shew_0820 at 943.926 kb on + strand, within Shew_0820 at 943.926 kb on + strand, within Shew_0820 at 943.934 kb on - strand, within Shew_0820 at 943.934 kb on - strand, within Shew_0820 at 944.183 kb on - strand, within Shew_0820 at 944.701 kb on + strand, within Shew_0820 at 945.195 kb on + strand, within Shew_0821 at 945.535 kb on + strand, within Shew_0822 at 945.758 kb on + strand, within Shew_0822
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 942,721 - Shew_0818 0.75 +0.1 942,896 + Shew_0818 0.84 +1.0 943,632 - -0.6 943,666 + -1.6 943,732 - +0.4 943,825 - Shew_0820 0.14 -0.3 943,926 + Shew_0820 0.22 -0.1 943,926 + Shew_0820 0.22 +1.0 943,926 + Shew_0820 0.22 +1.5 943,934 - Shew_0820 0.23 +0.1 943,934 - Shew_0820 0.23 +0.8 944,183 - Shew_0820 0.44 -0.9 944,701 + Shew_0820 0.87 +0.3 945,195 + Shew_0821 0.65 -1.1 945,535 + Shew_0822 0.29 +0.4 945,758 + Shew_0822 0.67 +0.1
Or see this region's nucleotide sequence