Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0540 and Shew_0541 are separated by 49 nucleotides Shew_0541 and Shew_0542 are separated by 12 nucleotides Shew_0542 and Shew_0543 are separated by 4 nucleotides Shew_0543 and tolC are separated by 67 nucleotides
Shew_0540: Shew_0540 - enoyl-CoA hydratase/isomerase (RefSeq), at 611,424 to 612,161
_0540
Shew_0541: Shew_0541 - hypothetical protein (RefSeq), at 612,211 to 612,678
_0541
Shew_0542: Shew_0542 - hypothetical protein (RefSeq), at 612,691 to 613,662
_0542
Shew_0543: Shew_0543 - hypothetical protein (RefSeq), at 613,667 to 614,425
_0543
Shew_0544: tolC - outer membrane channel protein (RefSeq), at 614,493 to 615,839
tolC
Position (kb) 612
613
614 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 611.817 kb on - strand, within Shew_0540 at 611.892 kb on - strand, within Shew_0540 at 612.080 kb on + strand, within Shew_0540 at 612.560 kb on + strand, within Shew_0541 at 612.689 kb on - strand at 612.704 kb on - strand at 612.785 kb on + strand at 612.785 kb on + strand at 612.819 kb on + strand, within Shew_0542 at 613.228 kb on + strand, within Shew_0542 at 613.295 kb on - strand, within Shew_0542 at 613.425 kb on - strand, within Shew_0542 at 613.490 kb on - strand, within Shew_0542 at 613.564 kb on - strand, within Shew_0542 at 613.581 kb on - strand at 613.702 kb on - strand at 614.025 kb on + strand, within Shew_0543 at 614.249 kb on - strand, within Shew_0543 at 614.414 kb on - strand at 614.637 kb on - strand, within tolC
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 611,817 - Shew_0540 0.53 +0.6 611,892 - Shew_0540 0.63 +1.2 612,080 + Shew_0540 0.89 -0.3 612,560 + Shew_0541 0.75 -0.4 612,689 - -1.3 612,704 - -1.3 612,785 + +0.0 612,785 + -0.9 612,819 + Shew_0542 0.13 -0.2 613,228 + Shew_0542 0.55 -0.1 613,295 - Shew_0542 0.62 -1.5 613,425 - Shew_0542 0.76 -0.5 613,490 - Shew_0542 0.82 -0.8 613,564 - Shew_0542 0.90 -0.9 613,581 - -1.9 613,702 - +0.3 614,025 + Shew_0543 0.47 +0.2 614,249 - Shew_0543 0.77 -0.8 614,414 - -0.2 614,637 - tolC Shew_0544 0.11 -4.7
Or see this region's nucleotide sequence