Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0293 and Shew_0294 are separated by 393 nucleotides Shew_0294 and Shew_0295 are separated by 80 nucleotides Shew_0295 and Shew_0296 are separated by 378 nucleotides
Shew_0293: Shew_0293 - threonine dehydratase (RefSeq), at 313,397 to 314,947
_0293
Shew_0294: Shew_0294 - alanine--glyoxylate transaminase (RefSeq), at 315,341 to 316,483
_0294
Shew_0295: Shew_0295 - hypothetical protein (RefSeq), at 316,564 to 316,989
_0295
Shew_0296: Shew_0296 - heat shock protein Hsp20 (RefSeq), at 317,368 to 317,838
_0296
Position (kb) 315
316
317 Strain fitness (log2 ratio) -1
0
1 at 314.595 kb on - strand, within Shew_0293 at 314.753 kb on - strand, within Shew_0293 at 314.834 kb on - strand at 314.834 kb on - strand at 314.900 kb on - strand at 315.240 kb on - strand at 315.335 kb on + strand at 315.365 kb on - strand at 315.554 kb on + strand, within Shew_0294 at 315.593 kb on - strand, within Shew_0294 at 316.006 kb on + strand, within Shew_0294 at 316.123 kb on + strand, within Shew_0294 at 316.259 kb on + strand, within Shew_0294 at 316.460 kb on + strand at 316.593 kb on + strand at 316.598 kb on + strand at 316.603 kb on + strand at 316.697 kb on - strand, within Shew_0295 at 316.705 kb on + strand, within Shew_0295 at 316.878 kb on + strand, within Shew_0295 at 316.922 kb on - strand, within Shew_0295 at 317.028 kb on + strand at 317.213 kb on + strand at 317.223 kb on + strand at 317.223 kb on + strand at 317.294 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 314,595 - Shew_0293 0.77 -0.6 314,753 - Shew_0293 0.87 +0.1 314,834 - +1.7 314,834 - -0.1 314,900 - -0.1 315,240 - +0.5 315,335 + +0.9 315,365 - +0.9 315,554 + Shew_0294 0.19 +0.4 315,593 - Shew_0294 0.22 +0.0 316,006 + Shew_0294 0.58 +0.5 316,123 + Shew_0294 0.68 -0.2 316,259 + Shew_0294 0.80 +1.2 316,460 + +0.2 316,593 + +0.3 316,598 + -0.0 316,603 + +0.8 316,697 - Shew_0295 0.31 -0.3 316,705 + Shew_0295 0.33 -0.1 316,878 + Shew_0295 0.74 -1.0 316,922 - Shew_0295 0.84 +1.1 317,028 + -1.4 317,213 + +0.4 317,223 + +0.2 317,223 + +0.6 317,294 + -1.4
Or see this region's nucleotide sequence