Experiment: Carbenicillin 50 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0022 and Shew_0023 overlap by 1 nucleotides Shew_0023 and Shew_0024 are separated by 21 nucleotides Shew_0024 and Shew_0025 are separated by 272 nucleotides
Shew_0022: Shew_0022 - TrkH family potassium uptake protein (RefSeq), at 25,599 to 27,056
_0022
Shew_0023: Shew_0023 - protoporphyrinogen oxidase (RefSeq), at 27,056 to 27,583
_0023
Shew_0024: Shew_0024 - ArsR family transcriptional regulator (RefSeq), at 27,605 to 27,910
_0024
Shew_0025: Shew_0025 - protoporphyrinogen oxidase (RefSeq), at 28,183 to 28,716
_0025
Position (kb) 27
28 Strain fitness (log2 ratio) -2
-1
0
1 at 26.182 kb on - strand, within Shew_0022 at 27.032 kb on - strand at 27.097 kb on + strand at 27.165 kb on + strand, within Shew_0023 at 27.173 kb on - strand, within Shew_0023 at 27.173 kb on - strand, within Shew_0023 at 27.208 kb on + strand, within Shew_0023 at 27.420 kb on - strand, within Shew_0023 at 27.540 kb on + strand at 27.556 kb on - strand at 27.567 kb on + strand at 27.594 kb on - strand at 27.671 kb on + strand, within Shew_0024 at 27.851 kb on - strand, within Shew_0024 at 28.301 kb on + strand, within Shew_0025 at 28.365 kb on - strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.442 kb on + strand, within Shew_0025
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 50 mg/ml remove 26,182 - Shew_0022 0.40 -1.2 27,032 - -1.4 27,097 + -0.7 27,165 + Shew_0023 0.21 -1.5 27,173 - Shew_0023 0.22 -1.2 27,173 - Shew_0023 0.22 -1.0 27,208 + Shew_0023 0.29 -0.6 27,420 - Shew_0023 0.69 +0.8 27,540 + -0.6 27,556 - -1.3 27,567 + -0.4 27,594 - +0.4 27,671 + Shew_0024 0.22 +1.0 27,851 - Shew_0024 0.80 -0.3 28,301 + Shew_0025 0.22 +0.5 28,365 - Shew_0025 0.34 -1.3 28,401 + Shew_0025 0.41 +0.3 28,401 + Shew_0025 0.41 -1.9 28,401 + Shew_0025 0.41 -1.1 28,442 + Shew_0025 0.49 -0.9
Or see this region's nucleotide sequence