Experiment: BHIS with Fusidic 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4207 and BT4208 are separated by 81 nucleotides BT4208 and BT4209 are separated by 9 nucleotides BT4209 and BT4210 are separated by 16 nucleotides
BT4207: BT4207 - UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase (NCBI ptt file), at 5,543,651 to 5,544,691
BT4207
BT4208: BT4208 - phosphohydrolase (NCBI ptt file), at 5,544,773 to 5,546,002
BT4208
BT4209: BT4209 - orotidine 5'-phosphate decarboxylase (NCBI ptt file), at 5,546,012 to 5,546,836
BT4209
BT4210: BT4210 - peptide chain release factor 1 (NCBI ptt file), at 5,546,853 to 5,547,965
BT4210
Position (kb)
5544
5545
5546
5547 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5544.775 kb on - strand at 5544.775 kb on - strand at 5544.775 kb on - strand at 5544.775 kb on - strand at 5544.802 kb on - strand at 5544.802 kb on - strand at 5544.802 kb on - strand at 5544.872 kb on - strand at 5544.886 kb on - strand at 5544.890 kb on - strand at 5544.893 kb on - strand at 5544.919 kb on - strand, within BT4208 at 5544.919 kb on - strand, within BT4208 at 5544.919 kb on - strand, within BT4208 at 5544.919 kb on - strand, within BT4208 at 5544.919 kb on - strand, within BT4208 at 5545.014 kb on - strand, within BT4208 at 5545.085 kb on - strand, within BT4208 at 5545.122 kb on - strand, within BT4208 at 5545.219 kb on - strand, within BT4208 at 5545.219 kb on - strand, within BT4208 at 5545.301 kb on - strand at 5545.486 kb on - strand, within BT4208 at 5545.598 kb on - strand, within BT4208 at 5545.679 kb on - strand, within BT4208 at 5545.739 kb on + strand, within BT4208 at 5545.764 kb on - strand, within BT4208 at 5545.766 kb on - strand, within BT4208 at 5545.766 kb on - strand, within BT4208 at 5545.843 kb on + strand, within BT4208 at 5545.844 kb on - strand, within BT4208 at 5545.844 kb on - strand, within BT4208 at 5545.862 kb on + strand, within BT4208 at 5545.863 kb on - strand, within BT4208 at 5545.863 kb on - strand, within BT4208 at 5545.881 kb on - strand at 5545.928 kb on - strand at 5545.983 kb on - strand at 5545.995 kb on + strand at 5546.237 kb on - strand, within BT4209 at 5546.288 kb on - strand, within BT4209 at 5546.406 kb on - strand, within BT4209 at 5546.464 kb on - strand, within BT4209 at 5546.518 kb on - strand, within BT4209 at 5546.841 kb on - strand at 5546.841 kb on - strand at 5546.841 kb on - strand at 5546.843 kb on - strand at 5546.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.002 mg/ml remove 5,544,775 - +1.9 5,544,775 - -0.1 5,544,775 - -0.9 5,544,775 - +0.9 5,544,802 - +0.7 5,544,802 - -3.4 5,544,802 - +1.7 5,544,872 - +2.2 5,544,886 - +1.7 5,544,890 - +0.8 5,544,893 - -0.2 5,544,919 - BT4208 0.12 +2.4 5,544,919 - BT4208 0.12 +2.4 5,544,919 - BT4208 0.12 -1.8 5,544,919 - BT4208 0.12 +1.0 5,544,919 - BT4208 0.12 +1.9 5,545,014 - BT4208 0.20 +2.6 5,545,085 - BT4208 0.25 +1.7 5,545,122 - BT4208 0.28 -1.5 5,545,219 - BT4208 0.36 +0.2 5,545,219 - BT4208 0.36 +1.1 5,545,301 - +0.1 5,545,486 - BT4208 0.58 +2.2 5,545,598 - BT4208 0.67 +2.5 5,545,679 - BT4208 0.74 +2.4 5,545,739 + BT4208 0.79 -1.3 5,545,764 - BT4208 0.81 +1.9 5,545,766 - BT4208 0.81 +1.3 5,545,766 - BT4208 0.81 +2.1 5,545,843 + BT4208 0.87 -2.5 5,545,844 - BT4208 0.87 -0.9 5,545,844 - BT4208 0.87 -0.6 5,545,862 + BT4208 0.89 -1.0 5,545,863 - BT4208 0.89 +2.3 5,545,863 - BT4208 0.89 -0.5 5,545,881 - +1.1 5,545,928 - +1.5 5,545,983 - -1.8 5,545,995 + -1.2 5,546,237 - BT4209 0.27 -2.2 5,546,288 - BT4209 0.33 -1.9 5,546,406 - BT4209 0.48 +0.1 5,546,464 - BT4209 0.55 -0.8 5,546,518 - BT4209 0.61 -0.2 5,546,841 - -0.3 5,546,841 - +1.5 5,546,841 - -0.0 5,546,843 - -2.2 5,546,846 - -1.6
Or see this region's nucleotide sequence