Experiment: BHIS with Fusidic 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2607 and BT2608 overlap by 13 nucleotides BT2608 and BT2609 are separated by 6 nucleotides BT2609 and BT2610 are separated by 87 nucleotides BT2610 and BT2611 are separated by 109 nucleotides BT2611 and BT2612 are separated by 2 nucleotides
BT2607: BT2607 - hypothetical protein (NCBI ptt file), at 3,248,177 to 3,248,851
BT2607
BT2608: BT2608 - hypothetical protein (NCBI ptt file), at 3,248,839 to 3,249,303
BT2608
BT2609: BT2609 - conserved protein found in conjugate transposon (NCBI ptt file), at 3,249,310 to 3,250,068
BT2609
BT2610: BT2610 - hypothetical protein (NCBI ptt file), at 3,250,156 to 3,250,581
BT2610
BT2611: BT2611 - hypothetical protein (NCBI ptt file), at 3,250,691 to 3,250,915
BT2611
BT2612: BT2612 - hypothetical protein (NCBI ptt file), at 3,250,918 to 3,251,340
BT2612
Position (kb)
3249
3250
3251 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3248.354 kb on - strand, within BT2607 at 3248.405 kb on - strand, within BT2607 at 3248.412 kb on - strand, within BT2607 at 3248.444 kb on + strand, within BT2607 at 3248.475 kb on + strand, within BT2607 at 3248.536 kb on + strand, within BT2607 at 3248.537 kb on - strand, within BT2607 at 3248.576 kb on + strand, within BT2607 at 3248.612 kb on - strand, within BT2607 at 3248.612 kb on - strand, within BT2607 at 3248.613 kb on + strand, within BT2607 at 3248.613 kb on + strand, within BT2607 at 3248.790 kb on - strand at 3248.794 kb on + strand at 3248.795 kb on - strand at 3248.796 kb on + strand at 3248.797 kb on - strand at 3248.797 kb on - strand at 3248.798 kb on + strand at 3248.798 kb on + strand at 3248.801 kb on + strand at 3248.802 kb on - strand at 3248.803 kb on + strand at 3248.835 kb on + strand at 3248.840 kb on + strand at 3248.896 kb on + strand, within BT2608 at 3248.897 kb on - strand, within BT2608 at 3248.907 kb on - strand, within BT2608 at 3248.929 kb on + strand, within BT2608 at 3248.998 kb on - strand, within BT2608 at 3248.999 kb on + strand, within BT2608 at 3249.013 kb on + strand, within BT2608 at 3249.013 kb on + strand, within BT2608 at 3249.014 kb on - strand, within BT2608 at 3249.014 kb on - strand, within BT2608 at 3249.016 kb on - strand, within BT2608 at 3249.017 kb on + strand, within BT2608 at 3249.017 kb on + strand, within BT2608 at 3249.017 kb on + strand, within BT2608 at 3249.017 kb on + strand, within BT2608 at 3249.018 kb on - strand, within BT2608 at 3249.018 kb on - strand, within BT2608 at 3249.084 kb on - strand, within BT2608 at 3249.229 kb on - strand, within BT2608 at 3249.272 kb on + strand at 3249.272 kb on + strand at 3249.297 kb on + strand at 3249.323 kb on - strand at 3249.406 kb on + strand at 3249.435 kb on + strand, within BT2609 at 3249.462 kb on + strand, within BT2609 at 3249.504 kb on - strand, within BT2609 at 3249.512 kb on + strand, within BT2609 at 3249.512 kb on + strand, within BT2609 at 3249.513 kb on - strand, within BT2609 at 3249.543 kb on + strand, within BT2609 at 3249.555 kb on + strand, within BT2609 at 3249.556 kb on - strand, within BT2609 at 3249.556 kb on - strand, within BT2609 at 3249.709 kb on - strand, within BT2609 at 3249.747 kb on - strand, within BT2609 at 3249.768 kb on + strand, within BT2609 at 3249.776 kb on + strand, within BT2609 at 3249.936 kb on - strand, within BT2609 at 3250.068 kb on + strand at 3250.089 kb on + strand at 3250.121 kb on - strand at 3250.121 kb on - strand at 3250.143 kb on - strand at 3250.146 kb on - strand at 3250.147 kb on + strand at 3250.161 kb on + strand at 3250.171 kb on + strand at 3250.177 kb on + strand at 3250.185 kb on + strand at 3250.185 kb on + strand at 3250.350 kb on + strand, within BT2610 at 3250.350 kb on + strand, within BT2610 at 3250.350 kb on + strand, within BT2610 at 3250.362 kb on + strand, within BT2610 at 3250.364 kb on + strand, within BT2610 at 3250.399 kb on + strand, within BT2610 at 3250.476 kb on + strand, within BT2610 at 3250.476 kb on + strand, within BT2610 at 3250.570 kb on + strand at 3250.575 kb on + strand at 3250.575 kb on + strand at 3250.575 kb on + strand at 3250.575 kb on + strand at 3250.579 kb on + strand at 3250.811 kb on - strand, within BT2611 at 3250.903 kb on - strand at 3250.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.002 mg/ml remove 3,248,354 - BT2607 0.26 +0.2 3,248,405 - BT2607 0.34 +0.2 3,248,412 - BT2607 0.35 -1.4 3,248,444 + BT2607 0.40 -1.2 3,248,475 + BT2607 0.44 +0.9 3,248,536 + BT2607 0.53 -1.4 3,248,537 - BT2607 0.53 +0.9 3,248,576 + BT2607 0.59 +0.8 3,248,612 - BT2607 0.64 -0.9 3,248,612 - BT2607 0.64 +0.5 3,248,613 + BT2607 0.65 +3.2 3,248,613 + BT2607 0.65 +1.6 3,248,790 - -0.8 3,248,794 + +4.0 3,248,795 - -2.0 3,248,796 + -0.3 3,248,797 - -0.2 3,248,797 - +0.0 3,248,798 + -0.2 3,248,798 + -1.5 3,248,801 + -0.6 3,248,802 - +0.8 3,248,803 + +2.0 3,248,835 + -1.8 3,248,840 + +0.6 3,248,896 + BT2608 0.12 +0.6 3,248,897 - BT2608 0.12 -2.3 3,248,907 - BT2608 0.15 -2.3 3,248,929 + BT2608 0.19 -1.5 3,248,998 - BT2608 0.34 -0.3 3,248,999 + BT2608 0.34 +0.0 3,249,013 + BT2608 0.37 +0.1 3,249,013 + BT2608 0.37 +0.7 3,249,014 - BT2608 0.38 -2.3 3,249,014 - BT2608 0.38 -0.4 3,249,016 - BT2608 0.38 +0.6 3,249,017 + BT2608 0.38 -1.4 3,249,017 + BT2608 0.38 -1.2 3,249,017 + BT2608 0.38 +0.4 3,249,017 + BT2608 0.38 +0.0 3,249,018 - BT2608 0.38 -1.0 3,249,018 - BT2608 0.38 -1.1 3,249,084 - BT2608 0.53 -3.9 3,249,229 - BT2608 0.84 -0.8 3,249,272 + -1.4 3,249,272 + +2.6 3,249,297 + +0.5 3,249,323 - +0.5 3,249,406 + +1.6 3,249,435 + BT2609 0.16 +0.7 3,249,462 + BT2609 0.20 -2.7 3,249,504 - BT2609 0.26 +1.2 3,249,512 + BT2609 0.27 +0.9 3,249,512 + BT2609 0.27 +0.6 3,249,513 - BT2609 0.27 -1.0 3,249,543 + BT2609 0.31 -0.0 3,249,555 + BT2609 0.32 +1.6 3,249,556 - BT2609 0.32 -1.0 3,249,556 - BT2609 0.32 +0.8 3,249,709 - BT2609 0.53 -2.0 3,249,747 - BT2609 0.58 -1.1 3,249,768 + BT2609 0.60 +0.9 3,249,776 + BT2609 0.61 -1.2 3,249,936 - BT2609 0.82 +0.8 3,250,068 + -0.7 3,250,089 + -0.5 3,250,121 - -1.0 3,250,121 - -3.1 3,250,143 - -0.6 3,250,146 - -0.1 3,250,147 + -2.1 3,250,161 + +1.6 3,250,171 + +0.6 3,250,177 + -2.2 3,250,185 + -0.2 3,250,185 + +0.5 3,250,350 + BT2610 0.46 +1.6 3,250,350 + BT2610 0.46 +0.1 3,250,350 + BT2610 0.46 -0.2 3,250,362 + BT2610 0.48 -2.9 3,250,364 + BT2610 0.49 -1.5 3,250,399 + BT2610 0.57 -0.8 3,250,476 + BT2610 0.75 -2.3 3,250,476 + BT2610 0.75 +1.3 3,250,570 + +2.4 3,250,575 + +0.5 3,250,575 + -2.7 3,250,575 + -0.7 3,250,575 + +0.2 3,250,579 + +0.0 3,250,811 - BT2611 0.53 -0.1 3,250,903 - -2.9 3,250,903 - -1.8
Or see this region's nucleotide sequence