Experiment: BHIS with Fusidic 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0832 and BT0833 are separated by 267 nucleotides BT0833 and BT0834 are separated by 178 nucleotides
BT0832: BT0832 - putative phosphoserine phosphatase (NCBI ptt file), at 1,027,033 to 1,028,262
BT0832
BT0833: BT0833 - conserved hypothetical protein (NCBI ptt file), at 1,028,530 to 1,029,036
BT0833
BT0834: BT0834 - conserved hypothetical protein (NCBI ptt file), at 1,029,215 to 1,030,312
BT0834
Position (kb)
1028
1029
1030 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1027.589 kb on - strand, within BT0832 at 1027.600 kb on + strand, within BT0832 at 1027.691 kb on + strand, within BT0832 at 1027.691 kb on + strand, within BT0832 at 1027.698 kb on + strand, within BT0832 at 1027.699 kb on - strand, within BT0832 at 1027.957 kb on + strand, within BT0832 at 1027.959 kb on + strand, within BT0832 at 1027.959 kb on + strand, within BT0832 at 1027.981 kb on - strand, within BT0832 at 1027.981 kb on - strand, within BT0832 at 1028.070 kb on + strand, within BT0832 at 1028.168 kb on + strand at 1028.169 kb on - strand at 1028.180 kb on - strand at 1028.200 kb on - strand at 1028.490 kb on + strand at 1028.512 kb on + strand at 1028.539 kb on + strand at 1028.703 kb on + strand, within BT0833 at 1028.708 kb on - strand, within BT0833 at 1028.867 kb on + strand, within BT0833 at 1028.872 kb on - strand, within BT0833 at 1028.980 kb on - strand, within BT0833 at 1029.020 kb on + strand at 1029.020 kb on + strand at 1029.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.002 mg/ml remove 1,027,589 - BT0832 0.45 +0.9 1,027,600 + BT0832 0.46 +0.2 1,027,691 + BT0832 0.53 -0.2 1,027,691 + BT0832 0.53 -3.0 1,027,698 + BT0832 0.54 +1.3 1,027,699 - BT0832 0.54 -3.3 1,027,957 + BT0832 0.75 -0.1 1,027,959 + BT0832 0.75 -0.9 1,027,959 + BT0832 0.75 +0.2 1,027,981 - BT0832 0.77 +1.3 1,027,981 - BT0832 0.77 +0.7 1,028,070 + BT0832 0.84 -1.2 1,028,168 + -0.6 1,028,169 - -1.1 1,028,180 - -1.1 1,028,200 - -0.2 1,028,490 + -1.2 1,028,512 + +0.5 1,028,539 + -0.8 1,028,703 + BT0833 0.34 -1.7 1,028,708 - BT0833 0.35 -1.1 1,028,867 + BT0833 0.66 -2.1 1,028,872 - BT0833 0.67 -1.9 1,028,980 - BT0833 0.89 -3.5 1,029,020 + -2.2 1,029,020 + -2.6 1,029,021 - -0.1
Or see this region's nucleotide sequence