Experiment: BHIS with Fusidic 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0125 and BT0126 are separated by 580 nucleotides BT0126 and BT0127 are separated by 144 nucleotides
BT0125: BT0125 - NADH:ubiquinone oxidoreductase subunit (NCBI ptt file), at 114,979 to 116,886
BT0125
BT0126: BT0126 - hypothetical protein (NCBI ptt file), at 117,467 to 120,223
BT0126
BT0127: BT0127 - putative transmembrane protein (NCBI ptt file), at 120,368 to 123,661
BT0127
Position (kb)
117
118
119
120
121 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 116.488 kb on + strand, within BT0125 at 116.492 kb on + strand, within BT0125 at 116.580 kb on + strand, within BT0125 at 116.650 kb on + strand, within BT0125 at 116.650 kb on + strand, within BT0125 at 116.855 kb on - strand at 116.872 kb on - strand at 116.886 kb on + strand at 116.887 kb on - strand at 116.890 kb on + strand at 116.890 kb on + strand at 117.046 kb on + strand at 117.091 kb on + strand at 117.105 kb on + strand at 117.164 kb on - strand at 117.309 kb on + strand at 117.310 kb on - strand at 117.310 kb on - strand at 117.336 kb on + strand at 117.667 kb on - strand at 117.671 kb on - strand at 117.671 kb on - strand at 117.679 kb on + strand at 117.749 kb on + strand, within BT0126 at 118.082 kb on + strand, within BT0126 at 118.082 kb on + strand, within BT0126 at 118.082 kb on + strand, within BT0126 at 118.083 kb on - strand, within BT0126 at 118.083 kb on - strand, within BT0126 at 118.085 kb on - strand, within BT0126 at 118.085 kb on - strand, within BT0126 at 118.208 kb on + strand, within BT0126 at 118.226 kb on + strand, within BT0126 at 118.347 kb on - strand, within BT0126 at 118.349 kb on - strand, within BT0126 at 118.379 kb on - strand, within BT0126 at 118.413 kb on - strand, within BT0126 at 118.429 kb on + strand, within BT0126 at 118.533 kb on + strand, within BT0126 at 118.587 kb on - strand, within BT0126 at 118.682 kb on - strand, within BT0126 at 118.834 kb on - strand, within BT0126 at 118.913 kb on + strand, within BT0126 at 118.914 kb on - strand, within BT0126 at 118.918 kb on - strand, within BT0126 at 119.009 kb on - strand, within BT0126 at 119.023 kb on + strand, within BT0126 at 119.130 kb on - strand, within BT0126 at 119.133 kb on - strand, within BT0126 at 119.153 kb on - strand, within BT0126 at 119.201 kb on - strand, within BT0126 at 119.273 kb on + strand, within BT0126 at 119.274 kb on - strand, within BT0126 at 119.364 kb on - strand, within BT0126 at 119.453 kb on - strand, within BT0126 at 119.500 kb on - strand, within BT0126 at 119.538 kb on + strand, within BT0126 at 119.541 kb on - strand, within BT0126 at 119.546 kb on - strand, within BT0126 at 119.620 kb on + strand, within BT0126 at 119.621 kb on - strand, within BT0126 at 119.621 kb on - strand, within BT0126 at 119.720 kb on + strand, within BT0126 at 119.721 kb on - strand, within BT0126 at 119.723 kb on - strand, within BT0126 at 119.918 kb on - strand, within BT0126 at 120.222 kb on - strand at 120.249 kb on - strand at 120.249 kb on - strand at 120.296 kb on - strand at 120.311 kb on + strand at 120.344 kb on - strand at 120.364 kb on + strand at 120.387 kb on + strand at 120.405 kb on + strand at 120.405 kb on + strand at 120.474 kb on + strand at 120.475 kb on - strand at 120.475 kb on - strand at 120.529 kb on - strand at 120.537 kb on - strand at 120.544 kb on + strand at 120.545 kb on - strand at 120.559 kb on - strand at 120.566 kb on - strand at 120.576 kb on - strand at 120.655 kb on - strand at 120.713 kb on - strand, within BT0127 at 120.729 kb on + strand, within BT0127 at 120.790 kb on - strand, within BT0127 at 120.841 kb on - strand, within BT0127 at 120.861 kb on + strand, within BT0127 at 120.862 kb on - strand, within BT0127 at 120.866 kb on - strand, within BT0127 at 120.866 kb on - strand, within BT0127 at 120.992 kb on - strand, within BT0127 at 121.021 kb on + strand, within BT0127 at 121.044 kb on - strand, within BT0127 at 121.044 kb on - strand, within BT0127 at 121.047 kb on + strand, within BT0127 at 121.097 kb on - strand, within BT0127
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.002 mg/ml remove 116,488 + BT0125 0.79 -2.7 116,492 + BT0125 0.79 -1.6 116,580 + BT0125 0.84 +0.4 116,650 + BT0125 0.88 -0.0 116,650 + BT0125 0.88 +0.1 116,855 - -0.0 116,872 - -1.2 116,886 + -1.1 116,887 - -2.2 116,890 + -0.7 116,890 + -1.0 117,046 + -2.0 117,091 + +0.2 117,105 + +0.8 117,164 - -2.7 117,309 + -0.2 117,310 - +0.4 117,310 - -0.1 117,336 + -2.3 117,667 - +1.4 117,671 - -1.6 117,671 - -2.0 117,679 + -1.5 117,749 + BT0126 0.10 +1.4 118,082 + BT0126 0.22 -0.0 118,082 + BT0126 0.22 -1.1 118,082 + BT0126 0.22 +0.3 118,083 - BT0126 0.22 -0.3 118,083 - BT0126 0.22 -0.3 118,085 - BT0126 0.22 -2.5 118,085 - BT0126 0.22 -0.2 118,208 + BT0126 0.27 -0.9 118,226 + BT0126 0.28 -1.4 118,347 - BT0126 0.32 -2.4 118,349 - BT0126 0.32 -0.9 118,379 - BT0126 0.33 +1.4 118,413 - BT0126 0.34 -2.6 118,429 + BT0126 0.35 +1.8 118,533 + BT0126 0.39 -2.2 118,587 - BT0126 0.41 -0.5 118,682 - BT0126 0.44 -0.0 118,834 - BT0126 0.50 -1.4 118,913 + BT0126 0.52 -0.2 118,914 - BT0126 0.52 -1.5 118,918 - BT0126 0.53 -0.1 119,009 - BT0126 0.56 +0.4 119,023 + BT0126 0.56 -0.0 119,130 - BT0126 0.60 +0.2 119,133 - BT0126 0.60 -2.0 119,153 - BT0126 0.61 -1.6 119,201 - BT0126 0.63 +0.6 119,273 + BT0126 0.66 -0.7 119,274 - BT0126 0.66 -1.0 119,364 - BT0126 0.69 -0.5 119,453 - BT0126 0.72 -0.6 119,500 - BT0126 0.74 +0.6 119,538 + BT0126 0.75 -1.4 119,541 - BT0126 0.75 -1.1 119,546 - BT0126 0.75 +0.4 119,620 + BT0126 0.78 +0.0 119,621 - BT0126 0.78 +0.4 119,621 - BT0126 0.78 -0.3 119,720 + BT0126 0.82 -2.4 119,721 - BT0126 0.82 -0.6 119,723 - BT0126 0.82 -0.6 119,918 - BT0126 0.89 -3.6 120,222 - +2.1 120,249 - +0.4 120,249 - +0.4 120,296 - -0.6 120,311 + +1.1 120,344 - -1.6 120,364 + -2.3 120,387 + -2.3 120,405 + -0.4 120,405 + -0.8 120,474 + -1.6 120,475 - +1.5 120,475 - -2.0 120,529 - +1.8 120,537 - -0.3 120,544 + -1.4 120,545 - -1.0 120,559 - +0.3 120,566 - +0.7 120,576 - -1.8 120,655 - -0.6 120,713 - BT0127 0.10 -2.6 120,729 + BT0127 0.11 +1.4 120,790 - BT0127 0.13 +0.5 120,841 - BT0127 0.14 -1.2 120,861 + BT0127 0.15 +1.4 120,862 - BT0127 0.15 -1.4 120,866 - BT0127 0.15 -0.6 120,866 - BT0127 0.15 -2.8 120,992 - BT0127 0.19 +0.9 121,021 + BT0127 0.20 +0.4 121,044 - BT0127 0.21 +2.7 121,044 - BT0127 0.21 -0.0 121,047 + BT0127 0.21 -0.9 121,097 - BT0127 0.22 +0.1
Or see this region's nucleotide sequence