Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_04490

Experiment: Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_04485 and AO353_04490 are separated by 286 nucleotidesAO353_04490 and AO353_04495 are separated by 124 nucleotidesAO353_04495 and AO353_04500 are separated by 49 nucleotides AO353_04485: AO353_04485 - phosphoribosylformylglycinamidine synthase, at 964,756 to 968,652 _04485 AO353_04490: AO353_04490 - murein transglycosylase, at 968,939 to 970,399 _04490 AO353_04495: AO353_04495 - pyridoxine 5'-phosphate synthase, at 970,524 to 971,267 _04495 AO353_04500: AO353_04500 - DNA repair protein RecO, at 971,317 to 972,006 _04500 Position (kb) 968 969 970 971Strain fitness (log2 ratio) -2 -1 0 1 2at 968.734 kb on - strandat 968.811 kb on - strandat 968.877 kb on + strandat 968.877 kb on + strandat 968.877 kb on + strandat 968.894 kb on - strandat 968.927 kb on + strandat 968.927 kb on + strandat 968.927 kb on + strandat 968.927 kb on + strandat 968.927 kb on + strandat 968.928 kb on - strandat 968.928 kb on - strandat 968.928 kb on - strandat 968.929 kb on + strandat 968.929 kb on + strandat 969.025 kb on + strandat 969.026 kb on - strandat 969.026 kb on - strandat 969.026 kb on - strandat 969.094 kb on + strand, within AO353_04490at 969.098 kb on + strand, within AO353_04490at 969.098 kb on + strand, within AO353_04490at 969.098 kb on + strand, within AO353_04490at 969.098 kb on + strand, within AO353_04490at 969.098 kb on + strand, within AO353_04490at 969.098 kb on + strand, within AO353_04490at 969.098 kb on + strand, within AO353_04490at 969.099 kb on - strand, within AO353_04490at 969.099 kb on - strand, within AO353_04490at 969.134 kb on + strand, within AO353_04490at 969.134 kb on + strand, within AO353_04490at 969.135 kb on - strand, within AO353_04490at 969.308 kb on + strand, within AO353_04490at 969.308 kb on + strand, within AO353_04490at 969.308 kb on + strand, within AO353_04490at 969.308 kb on + strand, within AO353_04490at 969.309 kb on - strand, within AO353_04490at 969.335 kb on + strand, within AO353_04490at 969.335 kb on + strand, within AO353_04490at 969.335 kb on + strand, within AO353_04490at 969.335 kb on + strand, within AO353_04490at 969.336 kb on - strand, within AO353_04490at 969.336 kb on - strand, within AO353_04490at 969.446 kb on + strand, within AO353_04490at 969.446 kb on + strand, within AO353_04490at 969.447 kb on - strand, within AO353_04490at 969.447 kb on - strand, within AO353_04490at 969.569 kb on + strand, within AO353_04490at 969.569 kb on + strand, within AO353_04490at 969.569 kb on + strand, within AO353_04490at 969.569 kb on + strand, within AO353_04490at 969.569 kb on + strand, within AO353_04490at 969.570 kb on - strand, within AO353_04490at 969.570 kb on - strand, within AO353_04490at 969.570 kb on - strand, within AO353_04490at 969.570 kb on - strand, within AO353_04490at 969.577 kb on + strand, within AO353_04490at 969.577 kb on + strand, within AO353_04490at 969.577 kb on + strand, within AO353_04490at 969.725 kb on + strand, within AO353_04490at 969.726 kb on - strand, within AO353_04490at 969.726 kb on - strand, within AO353_04490at 969.726 kb on - strand, within AO353_04490at 969.744 kb on + strand, within AO353_04490at 969.745 kb on - strand, within AO353_04490at 969.752 kb on + strand, within AO353_04490at 969.753 kb on - strand, within AO353_04490at 969.764 kb on + strand, within AO353_04490at 969.765 kb on - strand, within AO353_04490at 969.765 kb on - strand, within AO353_04490at 969.803 kb on + strand, within AO353_04490at 969.803 kb on + strand, within AO353_04490at 969.804 kb on - strand, within AO353_04490at 970.022 kb on + strand, within AO353_04490at 970.022 kb on + strand, within AO353_04490at 970.022 kb on + strand, within AO353_04490at 970.022 kb on + strand, within AO353_04490at 970.023 kb on - strand, within AO353_04490at 970.023 kb on - strand, within AO353_04490at 970.023 kb on - strand, within AO353_04490at 970.023 kb on - strand, within AO353_04490at 970.089 kb on - strand, within AO353_04490at 970.089 kb on - strand, within AO353_04490at 970.089 kb on - strand, within AO353_04490at 970.208 kb on + strand, within AO353_04490at 970.208 kb on + strand, within AO353_04490at 970.224 kb on - strand, within AO353_04490at 970.277 kb on + strandat 970.278 kb on - strandat 970.278 kb on - strandat 970.280 kb on + strandat 970.280 kb on + strandat 970.280 kb on + strandat 970.280 kb on + strandat 970.397 kb on + strandat 970.398 kb on - strandat 971.307 kb on + strandat 971.307 kb on + strandat 971.308 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Lomefloxacin 0.001 mM
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968,734 - +2.3
968,811 - +1.1
968,877 + +0.2
968,877 + +0.9
968,877 + -0.8
968,894 - -1.7
968,927 + -0.7
968,927 + +0.5
968,927 + +1.5
968,927 + +0.3
968,927 + +1.7
968,928 - +0.8
968,928 - +2.3
968,928 - -1.7
968,929 + -2.1
968,929 + +0.2
969,025 + -0.8
969,026 - -2.2
969,026 - -0.4
969,026 - +0.2
969,094 + AO353_04490 0.11 -1.3
969,098 + AO353_04490 0.11 -1.3
969,098 + AO353_04490 0.11 -0.9
969,098 + AO353_04490 0.11 -1.6
969,098 + AO353_04490 0.11 -1.8
969,098 + AO353_04490 0.11 +0.7
969,098 + AO353_04490 0.11 +0.3
969,098 + AO353_04490 0.11 -1.7
969,099 - AO353_04490 0.11 -1.5
969,099 - AO353_04490 0.11 -1.6
969,134 + AO353_04490 0.13 +0.2
969,134 + AO353_04490 0.13 -0.9
969,135 - AO353_04490 0.13 -0.7
969,308 + AO353_04490 0.25 -1.0
969,308 + AO353_04490 0.25 -0.3
969,308 + AO353_04490 0.25 -1.7
969,308 + AO353_04490 0.25 +0.7
969,309 - AO353_04490 0.25 +0.5
969,335 + AO353_04490 0.27 -0.7
969,335 + AO353_04490 0.27 -0.3
969,335 + AO353_04490 0.27 -0.8
969,335 + AO353_04490 0.27 -2.7
969,336 - AO353_04490 0.27 +0.7
969,336 - AO353_04490 0.27 +0.3
969,446 + AO353_04490 0.35 +0.5
969,446 + AO353_04490 0.35 -0.7
969,447 - AO353_04490 0.35 -2.1
969,447 - AO353_04490 0.35 -0.7
969,569 + AO353_04490 0.43 +1.7
969,569 + AO353_04490 0.43 -1.6
969,569 + AO353_04490 0.43 -1.6
969,569 + AO353_04490 0.43 +0.2
969,569 + AO353_04490 0.43 -1.8
969,570 - AO353_04490 0.43 +0.0
969,570 - AO353_04490 0.43 -1.8
969,570 - AO353_04490 0.43 -2.0
969,570 - AO353_04490 0.43 -1.5
969,577 + AO353_04490 0.44 +0.7
969,577 + AO353_04490 0.44 -1.0
969,577 + AO353_04490 0.44 -0.3
969,725 + AO353_04490 0.54 -1.8
969,726 - AO353_04490 0.54 -1.0
969,726 - AO353_04490 0.54 +1.5
969,726 - AO353_04490 0.54 -0.6
969,744 + AO353_04490 0.55 -1.0
969,745 - AO353_04490 0.55 -0.3
969,752 + AO353_04490 0.56 +0.6
969,753 - AO353_04490 0.56 -0.7
969,764 + AO353_04490 0.56 -1.3
969,765 - AO353_04490 0.57 +0.0
969,765 - AO353_04490 0.57 +0.3
969,803 + AO353_04490 0.59 -0.3
969,803 + AO353_04490 0.59 -1.1
969,804 - AO353_04490 0.59 -0.8
970,022 + AO353_04490 0.74 -1.6
970,022 + AO353_04490 0.74 +0.0
970,022 + AO353_04490 0.74 -1.1
970,022 + AO353_04490 0.74 -2.4
970,023 - AO353_04490 0.74 -0.8
970,023 - AO353_04490 0.74 -0.3
970,023 - AO353_04490 0.74 -1.1
970,023 - AO353_04490 0.74 +1.3
970,089 - AO353_04490 0.79 +0.0
970,089 - AO353_04490 0.79 -0.7
970,089 - AO353_04490 0.79 -2.1
970,208 + AO353_04490 0.87 -1.6
970,208 + AO353_04490 0.87 -2.7
970,224 - AO353_04490 0.88 -1.6
970,277 + +0.0
970,278 - -1.6
970,278 - -2.2
970,280 + -0.3
970,280 + +0.0
970,280 + +1.7
970,280 + -0.6
970,397 + -1.0
970,398 - +0.3
971,307 + -0.8
971,307 + -1.3
971,308 - +0.5

Or see this region's nucleotide sequence