Experiment: Vanillin 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt minC and Shewana3_2383 are separated by 27 nucleotides Shewana3_2383 and minE are separated by 2 nucleotides minE and Shewana3_2385 are separated by 162 nucleotides
Shewana3_2382: minC - septum formation inhibitor (RefSeq), at 2,861,806 to 2,862,471
minC
Shewana3_2383: Shewana3_2383 - septum site-determining protein MinD (RefSeq), at 2,862,499 to 2,863,308
_2383
Shewana3_2384: minE - cell division topological specificity factor MinE (RefSeq), at 2,863,311 to 2,863,568
minE
Shewana3_2385: Shewana3_2385 - ribonuclease D (RefSeq), at 2,863,731 to 2,864,897
_2385
Position (kb)
2863
2864 Strain fitness (log2 ratio)
-1
0
1 at 2862.399 kb on + strand, within minC at 2862.490 kb on + strand at 2862.525 kb on + strand at 2862.926 kb on + strand, within Shewana3_2383 at 2863.079 kb on + strand, within Shewana3_2383 at 2863.526 kb on + strand, within minE at 2863.669 kb on + strand at 2863.677 kb on - strand at 2864.253 kb on - strand, within Shewana3_2385 at 2864.386 kb on + strand, within Shewana3_2385 at 2864.388 kb on + strand, within Shewana3_2385 at 2864.487 kb on + strand, within Shewana3_2385 at 2864.495 kb on - strand, within Shewana3_2385 at 2864.550 kb on - strand, within Shewana3_2385
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 2.5 mM remove 2,862,399 + minC Shewana3_2382 0.89 +0.0 2,862,490 + +0.8 2,862,525 + -1.0 2,862,926 + Shewana3_2383 0.53 -0.2 2,863,079 + Shewana3_2383 0.72 -0.3 2,863,526 + minE Shewana3_2384 0.83 +0.8 2,863,669 + +0.5 2,863,677 - -0.3 2,864,253 - Shewana3_2385 0.45 -1.3 2,864,386 + Shewana3_2385 0.56 -0.8 2,864,388 + Shewana3_2385 0.56 +1.1 2,864,487 + Shewana3_2385 0.65 +1.5 2,864,495 - Shewana3_2385 0.65 +0.0 2,864,550 - Shewana3_2385 0.70 +1.5
Or see this region's nucleotide sequence