Experiment: Vanillin 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2062 and Shewana3_R0088 are separated by 80 nucleotides Shewana3_R0088 and Shewana3_2063 are separated by 403 nucleotides Shewana3_2063 and Shewana3_2064 are separated by 369 nucleotides
Shewana3_2062: Shewana3_2062 - inner membrane transport protein YdhC (RefSeq), at 2,467,952 to 2,469,223
_2062
Shewana3_R0088: Shewana3_R0088 - tRNA-Ser (RefSeq), at 2,469,304 to 2,469,394
_R0088
Shewana3_2063: Shewana3_2063 - hypothetical protein (RefSeq), at 2,469,798 to 2,470,328
_2063
Shewana3_2064: Shewana3_2064 - ribulokinase (RefSeq), at 2,470,698 to 2,472,371
_2064
Position (kb)
2469
2470
2471 Strain fitness (log2 ratio)
-1
0
1 at 2469.071 kb on + strand, within Shewana3_2062 at 2469.553 kb on + strand at 2469.869 kb on - strand, within Shewana3_2063 at 2470.007 kb on + strand, within Shewana3_2063 at 2470.037 kb on + strand, within Shewana3_2063 at 2470.196 kb on - strand, within Shewana3_2063 at 2470.220 kb on + strand, within Shewana3_2063 at 2470.324 kb on + strand at 2470.913 kb on - strand, within Shewana3_2064 at 2471.182 kb on - strand, within Shewana3_2064 at 2471.237 kb on - strand, within Shewana3_2064 at 2471.241 kb on + strand, within Shewana3_2064 at 2471.305 kb on - strand, within Shewana3_2064
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 2.5 mM remove 2,469,071 + Shewana3_2062 0.88 +0.5 2,469,553 + +0.3 2,469,869 - Shewana3_2063 0.13 +0.9 2,470,007 + Shewana3_2063 0.39 +0.1 2,470,037 + Shewana3_2063 0.45 +1.7 2,470,196 - Shewana3_2063 0.75 +0.1 2,470,220 + Shewana3_2063 0.79 +0.1 2,470,324 + +1.7 2,470,913 - Shewana3_2064 0.13 +1.7 2,471,182 - Shewana3_2064 0.29 -0.6 2,471,237 - Shewana3_2064 0.32 -0.3 2,471,241 + Shewana3_2064 0.32 -0.3 2,471,305 - Shewana3_2064 0.36 +1.7
Or see this region's nucleotide sequence