Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3130 and Shew_3131 are separated by 81 nucleotides Shew_3131 and Shew_3132 overlap by 1 nucleotides Shew_3132 and Shew_3133 are separated by 95 nucleotides
Shew_3130: Shew_3130 - glutamate--tRNA ligase (RefSeq), at 3,721,930 to 3,722,889
_3130
Shew_3131: Shew_3131 - poly(A) polymerase (RefSeq), at 3,722,971 to 3,724,413
_3131
Shew_3132: Shew_3132 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (RefSeq), at 3,724,413 to 3,724,901
_3132
Shew_3133: Shew_3133 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (RefSeq), at 3,724,997 to 3,725,791
_3133
Position (kb)
3722
3723
3724
3725 Strain fitness (log2 ratio)
-1
0
1 at 3722.022 kb on + strand at 3722.030 kb on - strand, within Shew_3130 at 3722.156 kb on + strand, within Shew_3130 at 3722.380 kb on + strand, within Shew_3130 at 3722.386 kb on - strand, within Shew_3130 at 3722.737 kb on + strand, within Shew_3130 at 3722.822 kb on - strand at 3722.901 kb on + strand at 3722.991 kb on + strand at 3723.576 kb on + strand, within Shew_3131 at 3723.992 kb on + strand, within Shew_3131 at 3724.239 kb on + strand, within Shew_3131 at 3724.310 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,722,022 + +0.2 3,722,030 - Shew_3130 0.10 +0.1 3,722,156 + Shew_3130 0.24 -0.1 3,722,380 + Shew_3130 0.47 -0.1 3,722,386 - Shew_3130 0.47 +0.1 3,722,737 + Shew_3130 0.84 +0.2 3,722,822 - -0.0 3,722,901 + -0.4 3,722,991 + +0.2 3,723,576 + Shew_3131 0.42 +1.0 3,723,992 + Shew_3131 0.71 +0.2 3,724,239 + Shew_3131 0.88 +0.4 3,724,310 + +0.5
Or see this region's nucleotide sequence