Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2421 and Shew_2422 are separated by 150 nucleotides Shew_2422 and Shew_2423 are separated by 18 nucleotides Shew_2423 and Shew_2424 are separated by 293 nucleotides Shew_2424 and fadJ are separated by 158 nucleotides
Shew_2421: Shew_2421 - diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq), at 2,843,433 to 2,847,746
_2421
Shew_2422: Shew_2422 - hypothetical protein (RefSeq), at 2,847,897 to 2,848,532
_2422
Shew_2423: Shew_2423 - hypothetical protein (RefSeq), at 2,848,551 to 2,848,670
_2423
Shew_2424: Shew_2424 - hypothetical protein (RefSeq), at 2,848,964 to 2,849,170
_2424
Shew_2425: fadJ - multifunctional fatty acid oxidation complex subunit alpha (RefSeq), at 2,849,329 to 2,851,449
fadJ
Position (kb)
2847
2848
2849 Strain fitness (log2 ratio)
-1
0
1 at 2846.981 kb on - strand, within Shew_2421 at 2846.981 kb on - strand, within Shew_2421 at 2846.997 kb on + strand, within Shew_2421 at 2847.100 kb on + strand, within Shew_2421 at 2847.408 kb on - strand at 2847.408 kb on + strand at 2847.527 kb on - strand at 2848.360 kb on - strand, within Shew_2422 at 2848.621 kb on - strand, within Shew_2423 at 2848.819 kb on - strand at 2848.855 kb on + strand at 2848.954 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,846,981 - Shew_2421 0.82 -0.3 2,846,981 - Shew_2421 0.82 -0.0 2,846,997 + Shew_2421 0.83 +0.2 2,847,100 + Shew_2421 0.85 -0.2 2,847,408 - -0.5 2,847,408 + +0.7 2,847,527 - -0.3 2,848,360 - Shew_2422 0.73 +0.6 2,848,621 - Shew_2423 0.58 +0.1 2,848,819 - +0.1 2,848,855 + -0.3 2,848,954 - +0.2
Or see this region's nucleotide sequence