Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2057 and Shew_2058 are separated by 159 nucleotides Shew_2058 and Shew_2059 are separated by 72 nucleotides Shew_2059 and Shew_R0071 are separated by 125 nucleotides Shew_R0071 and Shew_R0072 are separated by 31 nucleotides Shew_R0072 and Shew_R0073 are separated by 40 nucleotides Shew_R0073 and Shew_2060 are separated by 293 nucleotides
Shew_2057: Shew_2057 - diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq), at 2,375,454 to 2,377,643
_2057
Shew_2058: Shew_2058 - hypothetical protein (RefSeq), at 2,377,803 to 2,378,090
_2058
Shew_2059: Shew_2059 - hypothetical protein (RefSeq), at 2,378,163 to 2,378,651
_2059
Shew_R0071: Shew_R0071 - tRNA-Tyr (RefSeq), at 2,378,777 to 2,378,861
_R0071
Shew_R0072: Shew_R0072 - tRNA-Tyr (RefSeq), at 2,378,893 to 2,378,977
_R0072
Shew_R0073: Shew_R0073 - tRNA-Tyr (RefSeq), at 2,379,018 to 2,379,102
_R0073
Shew_2060: Shew_2060 - pyridoxal-5'-phosphate-dependent enzyme, beta subunit (RefSeq), at 2,379,396 to 2,380,457
_2060
Position (kb)
2378
2379 Strain fitness (log2 ratio)
-1
0
1 at 2377.802 kb on + strand at 2378.333 kb on - strand, within Shew_2059 at 2378.371 kb on - strand, within Shew_2059 at 2378.499 kb on - strand, within Shew_2059 at 2378.569 kb on - strand, within Shew_2059 at 2379.431 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,377,802 + +0.5 2,378,333 - Shew_2059 0.35 +1.1 2,378,371 - Shew_2059 0.43 +0.3 2,378,499 - Shew_2059 0.69 +0.6 2,378,569 - Shew_2059 0.83 +0.1 2,379,431 + -0.3
Or see this region's nucleotide sequence