Strain Fitness in Shewanella loihica PV-4 around Shew_2026

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShew_2024 and Shew_2025 are separated by 182 nucleotidesShew_2025 and Shew_2026 are separated by 179 nucleotidesShew_2026 and Shew_2027 are separated by 56 nucleotidesShew_2027 and Shew_2028 are separated by 131 nucleotides Shew_2024: Shew_2024 - cell divisionFtsK/SpoIIIE (RefSeq), at 2,336,656 to 2,339,181 _2024 Shew_2025: Shew_2025 - leucine-responsive transcriptional regulator (RefSeq), at 2,339,364 to 2,339,867 _2025 Shew_2026: Shew_2026 - alanine dehydrogenase (RefSeq), at 2,340,047 to 2,341,162 _2026 Shew_2027: Shew_2027 - NAD(P) transhydrogenase subunit alpha (RefSeq), at 2,341,219 to 2,341,938 _2027 Shew_2028: Shew_2028 - thioredoxin reductase (RefSeq), at 2,342,070 to 2,343,020 _2028 Position (kb) 2340 2341 2342Strain fitness (log2 ratio) -1 0 1at 2339.270 kb on + strandat 2339.395 kb on + strandat 2339.465 kb on + strand, within Shew_2025at 2339.746 kb on - strand, within Shew_2025at 2339.750 kb on + strand, within Shew_2025at 2339.753 kb on - strand, within Shew_2025at 2339.758 kb on - strand, within Shew_2025at 2339.758 kb on - strand, within Shew_2025at 2340.200 kb on + strand, within Shew_2026at 2342.015 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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2,339,270 + +0.9
2,339,395 + +0.4
2,339,465 + Shew_2025 0.20 +0.5
2,339,746 - Shew_2025 0.76 +0.2
2,339,750 + Shew_2025 0.77 -0.2
2,339,753 - Shew_2025 0.77 -0.4
2,339,758 - Shew_2025 0.78 -0.7
2,339,758 - Shew_2025 0.78 +0.3
2,340,200 + Shew_2026 0.14 +0.8
2,342,015 - +0.1

Or see this region's nucleotide sequence