Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1865 and Shew_1866 are separated by 11 nucleotides Shew_1866 and Shew_1867 are separated by 31 nucleotides Shew_1867 and Shew_1868 are separated by 215 nucleotides
Shew_1865: Shew_1865 - phosphoenolpyruvate-protein phosphotransferase (RefSeq), at 2,143,217 to 2,144,923
_1865
Shew_1866: Shew_1866 - phosphotransferase system, phosphocarrier protein HPr (RefSeq), at 2,144,935 to 2,145,192
_1866
Shew_1867: Shew_1867 - hypothetical protein (RefSeq), at 2,145,224 to 2,145,616
_1867
Shew_1868: Shew_1868 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 2,145,832 to 2,147,469
_1868
Position (kb)
2145
2146 Strain fitness (log2 ratio)
-1
0
1 at 2144.293 kb on + strand, within Shew_1865 at 2144.483 kb on - strand, within Shew_1865 at 2145.145 kb on + strand, within Shew_1866 at 2145.418 kb on + strand, within Shew_1867 at 2145.546 kb on + strand, within Shew_1867 at 2145.685 kb on + strand at 2145.874 kb on + strand at 2146.052 kb on - strand, within Shew_1868 at 2146.370 kb on - strand, within Shew_1868 at 2146.461 kb on - strand, within Shew_1868
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,144,293 + Shew_1865 0.63 +0.7 2,144,483 - Shew_1865 0.74 +1.3 2,145,145 + Shew_1866 0.81 +0.3 2,145,418 + Shew_1867 0.49 +0.5 2,145,546 + Shew_1867 0.82 +0.7 2,145,685 + +0.3 2,145,874 + +0.9 2,146,052 - Shew_1868 0.13 -0.8 2,146,370 - Shew_1868 0.33 +0.5 2,146,461 - Shew_1868 0.38 +0.3
Or see this region's nucleotide sequence