Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_R0055 and Shew_R0056 are separated by 39 nucleotides Shew_R0056 and Shew_1530 are separated by 656 nucleotides Shew_1530 and grxA are separated by 53 nucleotides grxA and Shew_1532 are separated by 65 nucleotides
Shew_R0055: Shew_R0055 - tRNA-Lys (RefSeq), at 1,753,864 to 1,753,939
_R0055
Shew_R0056: Shew_R0056 - tRNA-Lys (RefSeq), at 1,753,979 to 1,754,054
_R0056
Shew_1530: Shew_1530 - porin (RefSeq), at 1,754,711 to 1,755,853
_1530
Shew_1531: grxA - glutaredoxin 1 (RefSeq), at 1,755,907 to 1,756,164
grxA
Shew_1532: Shew_1532 - type III restriction enzyme, res subunit (RefSeq), at 1,756,230 to 1,757,969
_1532
Position (kb) 1754
1755
1756 Strain fitness (log2 ratio) -1
0
1 at 1754.182 kb on + strand at 1754.190 kb on - strand at 1754.335 kb on + strand at 1754.617 kb on + strand at 1754.799 kb on - strand at 1754.815 kb on + strand at 1754.996 kb on - strand, within Shew_1530 at 1755.354 kb on - strand, within Shew_1530 at 1755.730 kb on + strand, within Shew_1530 at 1755.756 kb on - strand at 1755.767 kb on + strand at 1756.001 kb on + strand, within grxA at 1756.124 kb on - strand, within grxA at 1756.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,754,182 + -1.6 1,754,190 - +0.4 1,754,335 + -0.5 1,754,617 + -1.1 1,754,799 - -1.0 1,754,815 + +0.1 1,754,996 - Shew_1530 0.25 +1.3 1,755,354 - Shew_1530 0.56 +0.5 1,755,730 + Shew_1530 0.89 +0.3 1,755,756 - +0.3 1,755,767 + -0.9 1,756,001 + grxA Shew_1531 0.36 -0.3 1,756,124 - grxA Shew_1531 0.84 +1.0 1,756,206 - +0.4
Or see this region's nucleotide sequence