Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1459 and Shew_1460 are separated by 84 nucleotides Shew_1460 and Shew_1461 are separated by 191 nucleotides Shew_1461 and Shew_1462 are separated by 68 nucleotides Shew_1462 and Shew_1463 are separated by 127 nucleotides
Shew_1459: Shew_1459 - hypothetical protein (RefSeq), at 1,682,087 to 1,682,491
_1459
Shew_1460: Shew_1460 - pseudouridine synthase (RefSeq), at 1,682,576 to 1,683,229
_1460
Shew_1461: Shew_1461 - zinc carboxypeptidase-related protein (RefSeq), at 1,683,421 to 1,684,398
_1461
Shew_1462: Shew_1462 - lysine exporter protein LysE/YggA (RefSeq), at 1,684,467 to 1,685,117
_1462
Shew_1463: Shew_1463 - AraC family transcriptional regulator (RefSeq), at 1,685,245 to 1,686,084
_1463
Position (kb)
1683
1684
1685 Strain fitness (log2 ratio)
-1
0
1 at 1683.194 kb on + strand at 1683.301 kb on + strand at 1683.513 kb on + strand at 1683.519 kb on + strand, within Shew_1461 at 1683.519 kb on + strand, within Shew_1461 at 1683.967 kb on - strand, within Shew_1461 at 1683.967 kb on - strand, within Shew_1461 at 1684.350 kb on + strand at 1684.689 kb on + strand, within Shew_1462 at 1684.725 kb on + strand, within Shew_1462 at 1684.755 kb on + strand, within Shew_1462 at 1684.763 kb on - strand, within Shew_1462 at 1684.934 kb on - strand, within Shew_1462 at 1685.053 kb on + strand at 1685.330 kb on - strand, within Shew_1463
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,683,194 + -0.3 1,683,301 + +0.6 1,683,513 + +0.1 1,683,519 + Shew_1461 0.10 +1.1 1,683,519 + Shew_1461 0.10 +0.3 1,683,967 - Shew_1461 0.56 +1.0 1,683,967 - Shew_1461 0.56 -0.1 1,684,350 + +0.0 1,684,689 + Shew_1462 0.34 +0.2 1,684,725 + Shew_1462 0.40 +0.5 1,684,755 + Shew_1462 0.44 +0.2 1,684,763 - Shew_1462 0.45 +0.5 1,684,934 - Shew_1462 0.72 +0.3 1,685,053 + +0.2 1,685,330 - Shew_1463 0.10 -0.2
Or see this region's nucleotide sequence