Experiment: Recovery broth with Ramoplanin 2e-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12985 and BBR_RS12990 are separated by 87 nucleotides BBR_RS12990 and BBR_RS12995 are separated by 105 nucleotides
BBR_RS12985: BBR_RS12985 - MFS transporter, at 683,285 to 684,604
_RS12985
BBR_RS12990: BBR_RS12990 - 4-hydroxy-tetrahydrodipicolinate reductase, at 684,692 to 685,447
_RS12990
BBR_RS12995: BBR_RS12995 - 4-hydroxy-tetrahydrodipicolinate synthase, at 685,553 to 686,458
_RS12995
Position (kb) 684
685
686 Strain fitness (log2 ratio) -2
-1
0
1
2 at 683.714 kb on - strand, within BBR_RS12985 at 683.754 kb on - strand, within BBR_RS12985 at 683.771 kb on - strand, within BBR_RS12985 at 683.881 kb on + strand, within BBR_RS12985 at 683.917 kb on + strand, within BBR_RS12985 at 683.941 kb on - strand, within BBR_RS12985 at 683.965 kb on - strand, within BBR_RS12985 at 683.975 kb on - strand, within BBR_RS12985 at 684.010 kb on + strand, within BBR_RS12985 at 684.027 kb on - strand, within BBR_RS12985 at 684.039 kb on - strand, within BBR_RS12985 at 684.053 kb on + strand, within BBR_RS12985 at 684.059 kb on + strand, within BBR_RS12985 at 684.073 kb on - strand, within BBR_RS12985 at 684.074 kb on + strand, within BBR_RS12985 at 684.108 kb on + strand, within BBR_RS12985 at 684.116 kb on - strand, within BBR_RS12985 at 684.133 kb on + strand, within BBR_RS12985 at 684.147 kb on + strand, within BBR_RS12985 at 684.155 kb on - strand, within BBR_RS12985 at 684.165 kb on + strand, within BBR_RS12985 at 684.204 kb on + strand, within BBR_RS12985 at 684.283 kb on + strand, within BBR_RS12985 at 684.283 kb on + strand, within BBR_RS12985 at 684.323 kb on - strand, within BBR_RS12985 at 684.323 kb on - strand, within BBR_RS12985 at 684.341 kb on - strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.431 kb on - strand, within BBR_RS12985 at 684.433 kb on + strand, within BBR_RS12985 at 684.441 kb on - strand, within BBR_RS12985 at 684.468 kb on - strand, within BBR_RS12985 at 684.497 kb on - strand at 684.497 kb on - strand at 684.550 kb on + strand at 684.550 kb on + strand at 684.550 kb on + strand at 684.552 kb on + strand at 684.558 kb on - strand at 684.573 kb on - strand at 684.584 kb on - strand at 684.599 kb on + strand at 684.599 kb on + strand at 684.607 kb on - strand at 684.607 kb on - strand at 684.694 kb on + strand at 684.735 kb on + strand at 684.749 kb on + strand at 684.749 kb on + strand at 684.803 kb on + strand, within BBR_RS12990 at 684.808 kb on + strand, within BBR_RS12990 at 684.831 kb on - strand, within BBR_RS12990 at 684.856 kb on + strand, within BBR_RS12990 at 684.874 kb on - strand, within BBR_RS12990 at 684.879 kb on - strand, within BBR_RS12990 at 684.887 kb on - strand, within BBR_RS12990 at 684.908 kb on - strand, within BBR_RS12990 at 684.925 kb on + strand, within BBR_RS12990 at 684.926 kb on - strand, within BBR_RS12990 at 684.929 kb on - strand, within BBR_RS12990 at 684.952 kb on + strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.990 kb on + strand, within BBR_RS12990 at 684.997 kb on + strand, within BBR_RS12990 at 685.015 kb on - strand, within BBR_RS12990 at 685.077 kb on + strand, within BBR_RS12990 at 685.091 kb on + strand, within BBR_RS12990 at 685.096 kb on + strand, within BBR_RS12990 at 685.105 kb on + strand, within BBR_RS12990 at 685.113 kb on - strand, within BBR_RS12990 at 685.113 kb on - strand, within BBR_RS12990 at 685.123 kb on + strand, within BBR_RS12990 at 685.131 kb on - strand, within BBR_RS12990 at 685.139 kb on - strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.178 kb on - strand, within BBR_RS12990 at 685.229 kb on - strand, within BBR_RS12990 at 685.249 kb on + strand, within BBR_RS12990 at 685.257 kb on - strand, within BBR_RS12990 at 685.280 kb on - strand, within BBR_RS12990 at 685.280 kb on - strand, within BBR_RS12990 at 685.289 kb on - strand, within BBR_RS12990 at 685.297 kb on - strand, within BBR_RS12990 at 685.409 kb on + strand at 685.412 kb on - strand at 685.412 kb on - strand at 685.434 kb on + strand at 685.456 kb on - strand at 685.517 kb on + strand at 685.520 kb on + strand at 685.541 kb on + strand at 685.549 kb on + strand at 685.549 kb on + strand at 685.551 kb on + strand at 685.557 kb on - strand at 685.701 kb on + strand, within BBR_RS12995 at 685.701 kb on + strand, within BBR_RS12995 at 685.738 kb on + strand, within BBR_RS12995 at 685.764 kb on - strand, within BBR_RS12995 at 685.765 kb on + strand, within BBR_RS12995 at 685.784 kb on + strand, within BBR_RS12995 at 685.855 kb on + strand, within BBR_RS12995 at 685.859 kb on + strand, within BBR_RS12995 at 685.976 kb on + strand, within BBR_RS12995 at 686.008 kb on + strand, within BBR_RS12995 at 686.014 kb on + strand, within BBR_RS12995 at 686.014 kb on + strand, within BBR_RS12995 at 686.014 kb on + strand, within BBR_RS12995 at 686.014 kb on + strand, within BBR_RS12995 at 686.021 kb on + strand, within BBR_RS12995 at 686.021 kb on + strand, within BBR_RS12995 at 686.022 kb on + strand, within BBR_RS12995 at 686.050 kb on + strand, within BBR_RS12995 at 686.050 kb on + strand, within BBR_RS12995 at 686.050 kb on + strand, within BBR_RS12995 at 686.119 kb on + strand, within BBR_RS12995 at 686.327 kb on + strand, within BBR_RS12995 at 686.337 kb on + strand, within BBR_RS12995 at 686.421 kb on + strand at 686.442 kb on + strand at 686.442 kb on + strand at 686.442 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Ramoplanin 2e-05 mM remove 683,714 - BBR_RS12985 0.33 -0.2 683,754 - BBR_RS12985 0.36 +0.9 683,771 - BBR_RS12985 0.37 -0.7 683,881 + BBR_RS12985 0.45 -0.2 683,917 + BBR_RS12985 0.48 -0.7 683,941 - BBR_RS12985 0.50 -0.6 683,965 - BBR_RS12985 0.52 +0.4 683,975 - BBR_RS12985 0.52 +0.2 684,010 + BBR_RS12985 0.55 -1.6 684,027 - BBR_RS12985 0.56 -1.6 684,039 - BBR_RS12985 0.57 +0.4 684,053 + BBR_RS12985 0.58 +0.9 684,059 + BBR_RS12985 0.59 -1.2 684,073 - BBR_RS12985 0.60 -0.1 684,074 + BBR_RS12985 0.60 +0.3 684,108 + BBR_RS12985 0.62 +0.7 684,116 - BBR_RS12985 0.63 +1.2 684,133 + BBR_RS12985 0.64 -0.5 684,147 + BBR_RS12985 0.65 -1.7 684,155 - BBR_RS12985 0.66 -1.7 684,165 + BBR_RS12985 0.67 -0.6 684,204 + BBR_RS12985 0.70 -0.6 684,283 + BBR_RS12985 0.76 -0.3 684,283 + BBR_RS12985 0.76 +0.5 684,323 - BBR_RS12985 0.79 -0.9 684,323 - BBR_RS12985 0.79 -0.5 684,341 - BBR_RS12985 0.80 -1.2 684,428 + BBR_RS12985 0.87 -1.2 684,428 + BBR_RS12985 0.87 -0.2 684,428 + BBR_RS12985 0.87 +0.5 684,431 - BBR_RS12985 0.87 -0.1 684,433 + BBR_RS12985 0.87 -0.3 684,441 - BBR_RS12985 0.88 -0.2 684,468 - BBR_RS12985 0.90 -0.5 684,497 - +0.3 684,497 - +1.0 684,550 + -0.9 684,550 + +2.0 684,550 + -0.5 684,552 + -2.2 684,558 - +0.1 684,573 - -0.2 684,584 - +0.7 684,599 + +0.7 684,599 + -0.8 684,607 - +0.3 684,607 - +0.8 684,694 + -0.2 684,735 + +0.2 684,749 + -0.3 684,749 + +0.3 684,803 + BBR_RS12990 0.15 +0.0 684,808 + BBR_RS12990 0.15 -0.1 684,831 - BBR_RS12990 0.18 -0.3 684,856 + BBR_RS12990 0.22 +0.5 684,874 - BBR_RS12990 0.24 -0.0 684,879 - BBR_RS12990 0.25 -0.6 684,887 - BBR_RS12990 0.26 -0.3 684,908 - BBR_RS12990 0.29 -0.0 684,925 + BBR_RS12990 0.31 +0.3 684,926 - BBR_RS12990 0.31 -0.9 684,929 - BBR_RS12990 0.31 -0.3 684,952 + BBR_RS12990 0.34 +0.6 684,960 - BBR_RS12990 0.35 +0.8 684,960 - BBR_RS12990 0.35 +0.2 684,960 - BBR_RS12990 0.35 +0.6 684,960 - BBR_RS12990 0.35 +0.7 684,990 + BBR_RS12990 0.39 +1.7 684,997 + BBR_RS12990 0.40 -0.6 685,015 - BBR_RS12990 0.43 +0.2 685,077 + BBR_RS12990 0.51 +1.2 685,091 + BBR_RS12990 0.53 +0.9 685,096 + BBR_RS12990 0.53 -0.2 685,105 + BBR_RS12990 0.55 +1.3 685,113 - BBR_RS12990 0.56 -0.2 685,113 - BBR_RS12990 0.56 -0.1 685,123 + BBR_RS12990 0.57 -0.1 685,131 - BBR_RS12990 0.58 +0.2 685,139 - BBR_RS12990 0.59 +0.3 685,145 + BBR_RS12990 0.60 +0.9 685,145 + BBR_RS12990 0.60 -0.4 685,145 + BBR_RS12990 0.60 +1.1 685,145 + BBR_RS12990 0.60 -1.4 685,145 + BBR_RS12990 0.60 -1.0 685,153 - BBR_RS12990 0.61 +0.8 685,153 - BBR_RS12990 0.61 +0.4 685,153 - BBR_RS12990 0.61 +0.8 685,178 - BBR_RS12990 0.64 -0.1 685,229 - BBR_RS12990 0.71 +0.2 685,249 + BBR_RS12990 0.74 +0.5 685,257 - BBR_RS12990 0.75 +1.1 685,280 - BBR_RS12990 0.78 +0.3 685,280 - BBR_RS12990 0.78 -0.3 685,289 - BBR_RS12990 0.79 -0.1 685,297 - BBR_RS12990 0.80 +0.2 685,409 + -0.5 685,412 - +1.2 685,412 - -0.5 685,434 + -0.4 685,456 - +0.6 685,517 + -0.1 685,520 + +0.7 685,541 + +0.9 685,549 + +1.2 685,549 + +1.1 685,551 + +1.3 685,557 - -1.9 685,701 + BBR_RS12995 0.16 +0.1 685,701 + BBR_RS12995 0.16 +0.1 685,738 + BBR_RS12995 0.20 -0.4 685,764 - BBR_RS12995 0.23 +1.5 685,765 + BBR_RS12995 0.23 +0.4 685,784 + BBR_RS12995 0.25 +0.8 685,855 + BBR_RS12995 0.33 +0.3 685,859 + BBR_RS12995 0.34 +0.5 685,976 + BBR_RS12995 0.47 +0.4 686,008 + BBR_RS12995 0.50 +0.3 686,014 + BBR_RS12995 0.51 +0.0 686,014 + BBR_RS12995 0.51 -0.2 686,014 + BBR_RS12995 0.51 -1.6 686,014 + BBR_RS12995 0.51 -0.9 686,021 + BBR_RS12995 0.52 +0.2 686,021 + BBR_RS12995 0.52 +0.1 686,022 + BBR_RS12995 0.52 -0.7 686,050 + BBR_RS12995 0.55 +0.2 686,050 + BBR_RS12995 0.55 +0.3 686,050 + BBR_RS12995 0.55 +0.3 686,119 + BBR_RS12995 0.62 +0.1 686,327 + BBR_RS12995 0.85 +0.7 686,337 + BBR_RS12995 0.87 -0.3 686,421 + +0.9 686,442 + +0.2 686,442 + +0.4 686,442 + +0.9
Or see this region's nucleotide sequence