Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3213 and Shewana3_3214 are separated by 202 nucleotides Shewana3_3214 and Shewana3_3215 are separated by 22 nucleotides Shewana3_3215 and Shewana3_3216 overlap by 4 nucleotides Shewana3_3216 and obgE are separated by 27 nucleotides
Shewana3_3213: Shewana3_3213 - hypothetical protein (RefSeq), at 3,858,222 to 3,858,635
_3213
Shewana3_3214: Shewana3_3214 - dihydrofolate reductase (RefSeq), at 3,858,838 to 3,859,320
_3214
Shewana3_3215: Shewana3_3215 - hypothetical protein (RefSeq), at 3,859,343 to 3,859,816
_3215
Shewana3_3216: Shewana3_3216 - hypothetical protein (RefSeq), at 3,859,813 to 3,860,574
_3216
Shewana3_3217: obgE - GTPase ObgE (RefSeq), at 3,860,602 to 3,861,768
obgE
Position (kb)
3859
3860 Strain fitness (log2 ratio)
-1
0
1 at 3858.418 kb on - strand, within Shewana3_3213 at 3858.821 kb on + strand at 3859.467 kb on - strand, within Shewana3_3215 at 3859.570 kb on - strand, within Shewana3_3215 at 3859.937 kb on - strand, within Shewana3_3216 at 3860.205 kb on - strand, within Shewana3_3216 at 3860.409 kb on - strand, within Shewana3_3216 at 3860.463 kb on - strand, within Shewana3_3216 at 3860.539 kb on - strand at 3860.698 kb on - strand at 3860.728 kb on - strand, within obgE
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 3,858,418 - Shewana3_3213 0.47 -0.5 3,858,821 + -0.5 3,859,467 - Shewana3_3215 0.26 -1.3 3,859,570 - Shewana3_3215 0.48 +0.9 3,859,937 - Shewana3_3216 0.16 +1.3 3,860,205 - Shewana3_3216 0.51 -0.6 3,860,409 - Shewana3_3216 0.78 +0.0 3,860,463 - Shewana3_3216 0.85 +0.1 3,860,539 - +0.0 3,860,698 - +0.6 3,860,728 - obgE Shewana3_3217 0.11 +0.4
Or see this region's nucleotide sequence