Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2595 and Shewana3_2596 are separated by 138 nucleotides Shewana3_2596 and Shewana3_2597 are separated by 131 nucleotides Shewana3_2597 and Shewana3_2598 are separated by 311 nucleotides Shewana3_2598 and Shewana3_2599 are separated by 64 nucleotides
Shewana3_2595: Shewana3_2595 - carbon starvation protein CstA (RefSeq), at 3,099,057 to 3,100,511
_2595
Shewana3_2596: Shewana3_2596 - hypothetical protein (RefSeq), at 3,100,650 to 3,101,018
_2596
Shewana3_2597: Shewana3_2597 - L-sorbosone dehydrogenase (SNDH) (RefSeq), at 3,101,150 to 3,102,292
_2597
Shewana3_2598: Shewana3_2598 - hypothetical protein (RefSeq), at 3,102,604 to 3,103,050
_2598
Shewana3_2599: Shewana3_2599 - hypothetical protein (RefSeq), at 3,103,115 to 3,103,984
_2599
Position (kb)
3101
3102
3103 Strain fitness (log2 ratio)
-2
-1
0
1 at 3100.253 kb on + strand, within Shewana3_2595 at 3100.385 kb on - strand at 3100.434 kb on + strand at 3100.629 kb on + strand at 3100.637 kb on - strand at 3101.491 kb on - strand, within Shewana3_2597 at 3101.515 kb on - strand, within Shewana3_2597 at 3101.968 kb on + strand, within Shewana3_2597 at 3102.036 kb on - strand, within Shewana3_2597 at 3102.088 kb on - strand, within Shewana3_2597 at 3102.266 kb on - strand at 3102.565 kb on - strand at 3102.888 kb on + strand, within Shewana3_2598 at 3103.045 kb on + strand at 3103.053 kb on - strand at 3103.239 kb on - strand, within Shewana3_2599
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 3,100,253 + Shewana3_2595 0.82 -0.8 3,100,385 - -1.7 3,100,434 + -0.3 3,100,629 + +0.1 3,100,637 - +0.8 3,101,491 - Shewana3_2597 0.30 -0.8 3,101,515 - Shewana3_2597 0.32 -2.4 3,101,968 + Shewana3_2597 0.72 +0.9 3,102,036 - Shewana3_2597 0.78 +0.5 3,102,088 - Shewana3_2597 0.82 +0.9 3,102,266 - +0.1 3,102,565 - +1.1 3,102,888 + Shewana3_2598 0.64 -0.1 3,103,045 + +0.1 3,103,053 - +0.1 3,103,239 - Shewana3_2599 0.14 -0.5
Or see this region's nucleotide sequence